Mariana C Fabra, Anabella A Campagna, Nicolás Farnetano, Juan P Anchordoquy, Juan M Anchordoquy, Ana C Carranza-Martin, Cecilia C Furnus, Noelia Nikoloff
{"title":"在屠宰场和opu衍生的牛卵母细胞体外培养过程中补充α -硫辛酸可提高胚胎质量。","authors":"Mariana C Fabra, Anabella A Campagna, Nicolás Farnetano, Juan P Anchordoquy, Juan M Anchordoquy, Ana C Carranza-Martin, Cecilia C Furnus, Noelia Nikoloff","doi":"10.1016/j.anireprosci.2024.107667","DOIUrl":null,"url":null,"abstract":"<p><p>The Ovum Pick-Up (OPU) technique offers a rapid path to genetic improvement. Embryonic genome activation (EGA) is crucial for successful embryonic development and occurs in two phases: minor EGA (MN<sub>EGA</sub>) from 2-cell to 8-cell stages, and major EGA (MJ<sub>EGA</sub>) from 8-cell to 16-cell stages. Supplementation with alpha-lipoic acid (ALA) during MN<sub>EGA</sub> using abattoir derived-oocytes has been shown to enhance in vitro embryo production. Two experiments were carried out in the study at hand. Experiment I aimed to evaluate ALA effects on embryo development and quality during MJ<sub>EGA</sub> using abattoir-derived oocytes. Oocytes were treated with either a Control in vitro culture (IVC) medium or 2.5 µM ALA during MJ<sub>EGA</sub>. The ALA presented a trend to increase the blastocyst cell number on day 7 (P = 0.06), along with reducing ROS levels and increasing GSH levels (P ≤ 0.05). Experiment II aimed to verify whether the results from abattoir- derived oocytes could be extrapolated to OPU-derived oocytes. The treatments included a Control, 2.5 µM ALA during MN<sub>EGA</sub>, and 2.5 µM ALA during MJ<sub>EGA</sub>. The ALA supplementation during MJ<sub>EGA</sub> improved the total blastocyst rate on day 8 (P = 0.04), with a trend towards increased blastocyst cell number on day 7 (P = 0.09). No improvements were observed in MN<sub>EGA</sub>. Overall, ALA supplementation during MJ<sub>EGA</sub> is more effective than during MN<sub>EGA</sub> for improving the embryonic quality of OPU-derived oocytes.</p>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"272 ","pages":"107667"},"PeriodicalIF":2.2000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Alpha-Lipoic acid supplementation during in vitro culture of abattoir- and OPU-derived bovine oocytes improves embryonic quality.\",\"authors\":\"Mariana C Fabra, Anabella A Campagna, Nicolás Farnetano, Juan P Anchordoquy, Juan M Anchordoquy, Ana C Carranza-Martin, Cecilia C Furnus, Noelia Nikoloff\",\"doi\":\"10.1016/j.anireprosci.2024.107667\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The Ovum Pick-Up (OPU) technique offers a rapid path to genetic improvement. Embryonic genome activation (EGA) is crucial for successful embryonic development and occurs in two phases: minor EGA (MN<sub>EGA</sub>) from 2-cell to 8-cell stages, and major EGA (MJ<sub>EGA</sub>) from 8-cell to 16-cell stages. Supplementation with alpha-lipoic acid (ALA) during MN<sub>EGA</sub> using abattoir derived-oocytes has been shown to enhance in vitro embryo production. Two experiments were carried out in the study at hand. Experiment I aimed to evaluate ALA effects on embryo development and quality during MJ<sub>EGA</sub> using abattoir-derived oocytes. Oocytes were treated with either a Control in vitro culture (IVC) medium or 2.5 µM ALA during MJ<sub>EGA</sub>. The ALA presented a trend to increase the blastocyst cell number on day 7 (P = 0.06), along with reducing ROS levels and increasing GSH levels (P ≤ 0.05). Experiment II aimed to verify whether the results from abattoir- derived oocytes could be extrapolated to OPU-derived oocytes. The treatments included a Control, 2.5 µM ALA during MN<sub>EGA</sub>, and 2.5 µM ALA during MJ<sub>EGA</sub>. The ALA supplementation during MJ<sub>EGA</sub> improved the total blastocyst rate on day 8 (P = 0.04), with a trend towards increased blastocyst cell number on day 7 (P = 0.09). No improvements were observed in MN<sub>EGA</sub>. Overall, ALA supplementation during MJ<sub>EGA</sub> is more effective than during MN<sub>EGA</sub> for improving the embryonic quality of OPU-derived oocytes.</p>\",\"PeriodicalId\":7880,\"journal\":{\"name\":\"Animal Reproduction Science\",\"volume\":\"272 \",\"pages\":\"107667\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Reproduction Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.anireprosci.2024.107667\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/12/9 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Reproduction Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.anireprosci.2024.107667","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/9 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Alpha-Lipoic acid supplementation during in vitro culture of abattoir- and OPU-derived bovine oocytes improves embryonic quality.
The Ovum Pick-Up (OPU) technique offers a rapid path to genetic improvement. Embryonic genome activation (EGA) is crucial for successful embryonic development and occurs in two phases: minor EGA (MNEGA) from 2-cell to 8-cell stages, and major EGA (MJEGA) from 8-cell to 16-cell stages. Supplementation with alpha-lipoic acid (ALA) during MNEGA using abattoir derived-oocytes has been shown to enhance in vitro embryo production. Two experiments were carried out in the study at hand. Experiment I aimed to evaluate ALA effects on embryo development and quality during MJEGA using abattoir-derived oocytes. Oocytes were treated with either a Control in vitro culture (IVC) medium or 2.5 µM ALA during MJEGA. The ALA presented a trend to increase the blastocyst cell number on day 7 (P = 0.06), along with reducing ROS levels and increasing GSH levels (P ≤ 0.05). Experiment II aimed to verify whether the results from abattoir- derived oocytes could be extrapolated to OPU-derived oocytes. The treatments included a Control, 2.5 µM ALA during MNEGA, and 2.5 µM ALA during MJEGA. The ALA supplementation during MJEGA improved the total blastocyst rate on day 8 (P = 0.04), with a trend towards increased blastocyst cell number on day 7 (P = 0.09). No improvements were observed in MNEGA. Overall, ALA supplementation during MJEGA is more effective than during MNEGA for improving the embryonic quality of OPU-derived oocytes.
期刊介绍：
Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction.
The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques.
The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.
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