曼氏血吸虫可溶性卵抗原体外和体内抑制结直肠癌生长的研究。

IF 4.5 2区医学 Q2 IMMUNOLOGY

Journal of Microbiology Immunology and Infection Pub Date : 2024-11-30 DOI:10.1016/j.jmii.2024.11.009

Ho Yin Pekkle Lam , Ting-Ruei Liang , Shinn-Jong Jiang , Shih-Yi Peng

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引用次数: 0

摘要

背景：结直肠癌（CRC）是世界上第三大最常见的恶性疾病。由于宿主的免疫在调节肿瘤细胞的生长和进展中起着重要的作用，免疫疗法因此引起了人们对治疗癌症的极大兴趣。目前，科学家们已经研究了血吸虫源性可溶性卵抗原（SEA）在治疗一系列免疫相关疾病中的应用，这种抗原被认为是一种强免疫调节剂。方法：本研究采用体外细胞系、HCT-116、DLD-1以及小鼠异种移植模型，研究SEA对结直肠癌的抗肿瘤作用。采用迁移法、侵袭法、免疫印迹法等方法分析SEA的抗肿瘤作用。此外，采用qRT-PCR和ELISA方法鉴定sea处理的细胞和sea处理的异种移植小鼠的免疫谱。结果：SEA对HCT-116和DLD-1细胞的生长和进展有剂量依赖性抑制作用。这种抑制伴随着上皮-间质转化（EMT）、炎性体失活和细胞凋亡的减少。SEA还下调了CRC细胞中IL-4和IL-10的表达，这可能是其生长和进展受到抑制的原因。体内研究显示SEA具有类似的有益作用，局部给药25 μg SEA可显著抑制肿瘤细胞生长。SEA治疗还将宿主的免疫从促肿瘤反应转变为抗肿瘤反应。结论：SEA可能对结直肠癌有良好的治疗作用，进一步的研究可能为结直肠癌的治疗带来希望。

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Schistosoma mansoni soluble egg antigen suppresses colorectal cancer growth in vitro and in vivo

Background

Colorectal cancer (CRC) is the third most common malignant disease around the world. Because the hosts’ immunity plays a great part in regulating tumor cells' growth and progression, immunotherapies have therefore aroused great interest in treating cancers. Currently, scientists have investigated the use of Schistosoma-derived soluble egg antigens (SEA), which is known as a strong immune modulator, in treating a series of immune-related diseases.

Methods

In this study, we investigated the anti-tumor effect of SEA against CRC using in vitro cell lines, HCT-116 and DLD-1, as well as in vivo mouse xenograft model. Approaches such as migration assay, invasion assay, and western blotting were done to analyze the anti-tumor effect of SEA. Furthermore, qRT-PCR and ELISA were performed to identify the immune profile of SEA-treated cells as well as SEA-treated xenograft mice.

Results

In vitro studies suggested that SEA can dose-dependently inhibit the growth and progression of HCT-116 and DLD-1 cells. This inhibition was accompanied by a reduction of epithelial-mesenchymal transition (EMT), inflammasome inactivation, and apoptosis. SEA also downregulated the expression of IL-4 and IL-10 in the CRC cells, which may be the reason why their growth and progression were suppressed. In vivo studies showed a similar beneficial effect of SEA, as local administration of 25 μg SEA significantly inhibits tumor cell growth. SEA treatment also shifts the host's immunity from a pro-tumorigenic response to an anti-tumor response.

Conclusion

In conclusion, SEA may provide a beneficial effect against CRC, and further investigation may give promise in CRC treatment.

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来源期刊

Journal of Microbiology Immunology and Infection IMMUNOLOGY-INFECTIOUS DISEASES

CiteScore

15.90

自引率

5.40%

发文量

159

审稿时长

67 days

期刊介绍： Journal of Microbiology Immunology and Infection is an open access journal, committed to disseminating information on the latest trends and advances in microbiology, immunology, infectious diseases and parasitology. Article types considered include perspectives, review articles, original articles, brief reports and correspondence. With the aim of promoting effective and accurate scientific information, an expert panel of referees constitutes the backbone of the peer-review process in evaluating the quality and content of manuscripts submitted for publication.