在AOM/DSS小鼠结肠炎相关结直肠癌模型中,富含α -亚麻酸的紫苏籽油冷榨提取物可减缓肿瘤进展并恢复肠道微生物稳态。

IF 2.6 3区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
PLoS ONE Pub Date : 2024-12-09 eCollection Date: 2024-01-01 DOI:10.1371/journal.pone.0315172
Chawin Korsirikoon, Peerapa Techaniyom, Aikkarach Kettawan, Thanaporn Rungruang, Chanatip Metheetrairut, Pinidphon Prombutara, Aurawan Kringkasemsee Kettawan
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引用次数: 0

摘要

本研究探讨了膳食营养素,尤其是紫苏籽油(PSO)中富含的植物源欧米伽-3 脂肪酸α-亚麻酸(ALA)对结肠炎相关性结直肠癌(CRC)发病的影响。该研究采用小鼠模型,仔细研究了富含 ALA 的紫苏籽油对炎症驱动的 CRC 的影响。研究人员对紫苏籽进行了榨油,并分析了所获油的营养成分。雄性 ICR 小鼠在四周龄时开始饲喂含 5%、10% 或 20% PSO、10% 鱼油或 5% 大豆油的食物。除对照组(5% 大豆油)外,所有组均使用偶氮甲烷(AOM)和葡聚糖硫酸钠(DSS)诱导小鼠患上 CRC。对疾病发展、结肠样本、肿瘤前病变、发育不良和生物标志物进行了细致的评估。此外,还通过 16S rRNA 测序阐明了肠道微生物群的组成。分析结果显示,PSO 含有 61.32% 的 ALA 和 783.90 mg/kg 的生育酚。使用含 5%大豆油或 10%鱼油饮食的小鼠表现出更高的肿瘤发病率、负担、复发率和异常隐窝计数。值得注意的是,喂食 5% PSO 的小鼠的这些参数都明显降低。此外,喂食 5% PSO 的小鼠结肠组织中的增殖和促炎标记物减少,AOM/DSS 引起的肠道菌群失调也有所缓解。值得注意的是,在 AOM/DSS 小鼠模型中,PSO 通过抑制增殖和促炎蛋白水平以及缓解肠道菌群失调,对结肠炎相关的 CRC 起到了抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cold-pressed extraction of perilla seed oil enriched with alpha-linolenic acid mitigates tumour progression and restores gut microbial homeostasis in the AOM/DSS mice model of colitis-associated colorectal cancer.

The present investigation explores into the influence of dietary nutrients, particularly alpha-linolenic acid (ALA), a plant-derived omega-3 fatty acid abundant in perilla seed oil (PSO), on the development of colitis-associated colorectal cancer (CRC). The study employs a mouse model to scrutinize the effects of ALA-rich PSO in the context of inflammation-driven CRC. Perilla seeds were subjected to oil extraction, and the nutritional composition of the obtained oil was analysed. Male ICR mice, initiated at four weeks of age, were subjected to diets comprising 5%, 10%, or 20% PSO, 10% fish oil, or 5% soybean oil. All groups, with the exception of the control group (5% soybean oil), underwent induction with azoxymethane (AOM) and dextran sulphate sodium (DSS) to instigate CRC. Disease development, colon samples, preneoplastic lesions, dysplasia, and biomarkers were meticulously evaluated. Furthermore, gut microbiota composition was elucidated through 16S rRNA sequencing. The analysis revealed that PSO contained 61.32% ALA and 783.90 mg/kg tocopherols. Mice subjected to diets comprising 5% soybean or 10% fish oil exhibited higher tumour incidence, burden, multiplicity, and aberrant crypt counts. Remarkably, these parameters were significantly reduced in mice fed a 5% PSO diet. Additionally, 5% PSO-fed mice displayed reduced proliferative and pro-inflammatory markers in colon tissues, coupled with an alleviation of AOM/DSS-induced gut dysbiosis. Notably, PSO demonstrated inhibitory effects on colitis-associated CRC in the AOM/DSS mice model, achieved through the suppression of proliferative and pro-inflammatory protein levels, and mitigation of gut dysbiosis, with discernible efficacy observed at a 5% dietary concentration.

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来源期刊
PLoS ONE
PLoS ONE 生物-生物学
CiteScore
6.20
自引率
5.40%
发文量
14242
审稿时长
3.7 months
期刊介绍: PLOS ONE is an international, peer-reviewed, open-access, online publication. PLOS ONE welcomes reports on primary research from any scientific discipline. It provides: * Open-access—freely accessible online, authors retain copyright * Fast publication times * Peer review by expert, practicing researchers * Post-publication tools to indicate quality and impact * Community-based dialogue on articles * Worldwide media coverage
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