{"title":"基于脓毒症患者外周血细胞-细胞相互作用的预后核心基因筛选。","authors":"Shaolan Li, Wenhao Chen, Zhihong Zhang, Ling Yuan, Yingchun Hu, Muhu Chen","doi":"10.1515/biol-2022-0999","DOIUrl":null,"url":null,"abstract":"<p><p>Peripheral blood samples from 15 septic patients admitted within 24 h and 8 healthy volunteers were used to conduct RNA-seq. Quantitative PCR of THP1 cells was performed to investigate the expression levels of the selected key genes. A total of 1,128 differential genes were identified, 721 of which were upregulated and 407 were downregulated. These genes are mainly involved in neutrophil activation, T cell regulation, immune effector process regulation, cytokine receptor activity, and cytokine binding. The six target genes were ELANE, IL1R2, RAB13, RNASE3, FCGR1A, and TLR5. In the sepsis group, FCGR1A and TLR5 were positively associated with survival compared to ELANE, IL1R2, RAB13, and RNASE3, which were adversely associated with survival. Furthermore, a meta-analysis based on public databases revealed an increased expression of these six target genes in the peripheral blood of patients with sepsis. In addition, we discovered that monocytes primarily express these genes. Using qPCR, we confirmed that these six important genes were highly expressed in lipopolysaccharide-treated THP1 cells. In summary, these findings suggest that ELANE, IL1R2, RAB13, RNASE3, FCGR1A, and TLR5 may influence the prognosis of patients with sepsis and provide novel insights and potential avenues for the treatment of sepsis.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"19 1","pages":"20220999"},"PeriodicalIF":1.7000,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11627055/pdf/","citationCount":"0","resultStr":"{\"title\":\"Screening of prognostic core genes based on cell-cell interaction in the peripheral blood of patients with sepsis.\",\"authors\":\"Shaolan Li, Wenhao Chen, Zhihong Zhang, Ling Yuan, Yingchun Hu, Muhu Chen\",\"doi\":\"10.1515/biol-2022-0999\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Peripheral blood samples from 15 septic patients admitted within 24 h and 8 healthy volunteers were used to conduct RNA-seq. Quantitative PCR of THP1 cells was performed to investigate the expression levels of the selected key genes. A total of 1,128 differential genes were identified, 721 of which were upregulated and 407 were downregulated. These genes are mainly involved in neutrophil activation, T cell regulation, immune effector process regulation, cytokine receptor activity, and cytokine binding. The six target genes were ELANE, IL1R2, RAB13, RNASE3, FCGR1A, and TLR5. In the sepsis group, FCGR1A and TLR5 were positively associated with survival compared to ELANE, IL1R2, RAB13, and RNASE3, which were adversely associated with survival. Furthermore, a meta-analysis based on public databases revealed an increased expression of these six target genes in the peripheral blood of patients with sepsis. In addition, we discovered that monocytes primarily express these genes. Using qPCR, we confirmed that these six important genes were highly expressed in lipopolysaccharide-treated THP1 cells. In summary, these findings suggest that ELANE, IL1R2, RAB13, RNASE3, FCGR1A, and TLR5 may influence the prognosis of patients with sepsis and provide novel insights and potential avenues for the treatment of sepsis.</p>\",\"PeriodicalId\":19605,\"journal\":{\"name\":\"Open Life Sciences\",\"volume\":\"19 1\",\"pages\":\"20220999\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-11-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11627055/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Open Life Sciences\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1515/biol-2022-0999\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Life Sciences","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1515/biol-2022-0999","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
Screening of prognostic core genes based on cell-cell interaction in the peripheral blood of patients with sepsis.
Peripheral blood samples from 15 septic patients admitted within 24 h and 8 healthy volunteers were used to conduct RNA-seq. Quantitative PCR of THP1 cells was performed to investigate the expression levels of the selected key genes. A total of 1,128 differential genes were identified, 721 of which were upregulated and 407 were downregulated. These genes are mainly involved in neutrophil activation, T cell regulation, immune effector process regulation, cytokine receptor activity, and cytokine binding. The six target genes were ELANE, IL1R2, RAB13, RNASE3, FCGR1A, and TLR5. In the sepsis group, FCGR1A and TLR5 were positively associated with survival compared to ELANE, IL1R2, RAB13, and RNASE3, which were adversely associated with survival. Furthermore, a meta-analysis based on public databases revealed an increased expression of these six target genes in the peripheral blood of patients with sepsis. In addition, we discovered that monocytes primarily express these genes. Using qPCR, we confirmed that these six important genes were highly expressed in lipopolysaccharide-treated THP1 cells. In summary, these findings suggest that ELANE, IL1R2, RAB13, RNASE3, FCGR1A, and TLR5 may influence the prognosis of patients with sepsis and provide novel insights and potential avenues for the treatment of sepsis.
期刊介绍:
Open Life Sciences (previously Central European Journal of Biology) is a fast growing peer-reviewed journal, devoted to scholarly research in all areas of life sciences, such as molecular biology, plant science, biotechnology, cell biology, biochemistry, biophysics, microbiology and virology, ecology, differentiation and development, genetics and many others. Open Life Sciences assures top quality of published data through critical peer review and editorial involvement throughout the whole publication process. Thanks to the Open Access model of publishing, it also offers unrestricted access to published articles for all users.