芽孢杆菌TTMP20转录组测序及BDH基因对四甲基吡嗪合成的影响分析

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2024-12-09 DOI:10.1007/s00284-024-03990-5

Shiyu Liang, Mengxiang Ling, Lihua Li, Shanling Gan, Changhua Shang

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引用次数: 0

摘要

芽孢杆菌TTMP20具有较高的四甲基吡嗪（TTMP）产率。然而，目前该菌株产生TTMP的机制尚不清楚，这限制了对菌株TTMP20的修饰。本研究确定了TTMP合成的关键代谢途径，包括糖酵解途径、三羧酸循环途径和氮代谢途径。构建了以PHY-300PLK-BDH为载体的TTMP20转基因芽孢杆菌。2,3-丁二醇脱氢酶基因（BDH）的过表达显著降低了TTMP的生物合成。本研究将为TTMP在关键酶基因水平上的生物合成调控提供新的思路。

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Transcriptome Sequencing of Bacillus sp. TTMP20 and Analysis of the Effect of BDH Gene on Tetramethylpyrazine Synthesis.

Bacillus sp. TTMP20 has high tetramethylpyrazine (TTMP) yield. However, the mechanism of TTMP production in this strain is unclear at present, which limits the modification for strain TTMP20. In this study, key metabolic pathways related to TTMP synthesis were identified, which included glycolytic pathway, tricarboxylic acid cycle and nitrogen metabolism pathway. Moreover, transgenic Bacillus sp. TTMP20 with recombinant vector PHY-300PLK-BDH was constructed. 2,3-butanediol dehydrogenase gene (BDH) overexpression considerably reduced TTMP biosynthesis. This study will supply new insight into the regulation of TTMP biosynthesis at key enzyme gene level.

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.