AllplexTM stx1/2/2a/2d分型（ASTXT）检测对产志贺毒素大肠杆菌诊断和分型的评价

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-12-03 DOI:10.1016/j.diagmicrobio.2024.116645

Hyeon Ji Lee , Hyeon-Joong Kim , Soon Seong Choi , Hye Jeong Choo , Hee Jae Huh , Eun Jeong Won

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引用次数: 0

摘要

本研究评估了AllplexTM stx1/2/2a/2d分型（ASTXT）检测（Seegene）对产志贺毒素大肠杆菌的诊断和分型的性能。对18株加标菌株进行分析敏感性和特异性评价，并对114株不含志贺毒素的大肠杆菌进行交叉反应性检测。临床表现评估使用熟练程度面板和76个粪便样本。菌株stx1、stx2、stx2a和stx2d的检测灵敏度分别为4.14 log10 CFU ml-1、4.47 log10 CFU ml-1、4.03 log10 CFU ml-1和4.02 log10 CFU ml-1。误差在5%以内，未观察到交叉反应。ASTXT检测显示与熟练程度小组结果完全一致，并且使用临床粪便样本达到100%阳性和阴性。ASTXT试验在快速检测和分型人类粪便样本中产志贺毒素大肠杆菌方面表现出可靠的性能。

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Evaluation of the AllplexTM stx1/2/2a/2d Typing (ASTXT) assay for the diagnosis and typing of Shiga toxin-producing Escherichia coli

This study assessed the performance of the Allplex^TM stx1/2/2a/2d Typing (ASTXT) assay (Seegene) for diagnosis and typing Shiga toxin-producing Escherichia coli. Analytical sensitivity and specificity were evaluated using 18 spiked strains, and cross-reactivity was tested on 114 strains including E. coli without Shiga toxin. Clinical performance was evaluated using the proficiency panels and 76 stool samples. Analytical sensitivity was revealed to 4.14 log₁₀ CFU ml⁻¹ for E. coli strain with stx1, 4.47 log₁₀ CFU ml⁻¹ for strain with stx2, 4.03 log₁₀ CFU ml⁻¹ for strain with stx2a, and 4.02 log₁₀ CFU ml⁻¹ for strain with stx2d, respectively. Imprecisions were within 5 % and cross-reactivity was not observed. The ASTXT assay showed perfect agreement with the proficiency panel results and achieved 100 % positive and negative percent agreement using clinical stool samples. The ASTXT assay demonstrated reliable performance for the rapid detection and typing of Shiga toxin-producing E. coli in human stool samples.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.