Gui-Min Kim, Yoojin Choi, Kyeong Rok Choi, Ilsong Lee, Jayeong Kim, Byunghyun Lee, Sang Yup Lee and Doh C. Lee
{"title":"光驱动合成氨的镍兰氏固氮菌体内半导体纳米颗粒合成","authors":"Gui-Min Kim, Yoojin Choi, Kyeong Rok Choi, Ilsong Lee, Jayeong Kim, Byunghyun Lee, Sang Yup Lee and Doh C. Lee","doi":"10.1039/D4NR02177K","DOIUrl":null,"url":null,"abstract":"<p >Ammonia (NH<small><sub>3</sub></small>) is an important commodity chemical used as an agricultural fertilizer and hydrogen-storage material. There has recently been much interest in developing an environmentally benign process for NH<small><sub>3</sub></small> synthesis. Here, we report enhanced production of ammonia from diazotrophs under light irradiation using hybrid composites of inorganic nanoparticles (NPs) and bacterial cells. The primary focus of this study lies in the intracellular biosynthesis of semiconductor NPs within <em>Azotobacter vinelandii</em>, a diazotroph, when bacterial cells are cultured in a medium containing precursor molecules. For example, enzymes in bacterial cells, such as cysteine desulfurase, convert cysteine (Cys) into precursors for cadmium sulfide (CdS) synthesis when supplied with CdCl<small><sub>2</sub></small>. Photoexcited charge carriers in the biosynthesized NPs are transferred to nitrogen fixation enzymes, <em>e.g.</em>, nitrogenase, facilitating the production of ammonium ions. Notably, the intracellular biosynthesis approach minimizes cell toxicity compared to extracellular synthesis due to the diminished generation of reactive oxygen species. The biohybrid system based on the <em>in vivo</em> approach results in a fivefold increase in ammonia production (0.45 mg g<small><sub>DCW</sub></small><small><sup>−1</sup></small> h<small><sup>−1</sup></small>) compared to the case of diazotroph cells only (0.09 mg g<small><sub>DCW</sub></small><small><sup>−1</sup></small> h<small><sup>−1</sup></small>).</p>","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":" 6","pages":" 3381-3388"},"PeriodicalIF":5.8000,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In vivo synthesis of semiconductor nanoparticles in Azotobacter vinelandii for light-driven ammonia production†\",\"authors\":\"Gui-Min Kim, Yoojin Choi, Kyeong Rok Choi, Ilsong Lee, Jayeong Kim, Byunghyun Lee, Sang Yup Lee and Doh C. Lee\",\"doi\":\"10.1039/D4NR02177K\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Ammonia (NH<small><sub>3</sub></small>) is an important commodity chemical used as an agricultural fertilizer and hydrogen-storage material. There has recently been much interest in developing an environmentally benign process for NH<small><sub>3</sub></small> synthesis. Here, we report enhanced production of ammonia from diazotrophs under light irradiation using hybrid composites of inorganic nanoparticles (NPs) and bacterial cells. The primary focus of this study lies in the intracellular biosynthesis of semiconductor NPs within <em>Azotobacter vinelandii</em>, a diazotroph, when bacterial cells are cultured in a medium containing precursor molecules. For example, enzymes in bacterial cells, such as cysteine desulfurase, convert cysteine (Cys) into precursors for cadmium sulfide (CdS) synthesis when supplied with CdCl<small><sub>2</sub></small>. Photoexcited charge carriers in the biosynthesized NPs are transferred to nitrogen fixation enzymes, <em>e.g.</em>, nitrogenase, facilitating the production of ammonium ions. Notably, the intracellular biosynthesis approach minimizes cell toxicity compared to extracellular synthesis due to the diminished generation of reactive oxygen species. The biohybrid system based on the <em>in vivo</em> approach results in a fivefold increase in ammonia production (0.45 mg g<small><sub>DCW</sub></small><small><sup>−1</sup></small> h<small><sup>−1</sup></small>) compared to the case of diazotroph cells only (0.09 mg g<small><sub>DCW</sub></small><small><sup>−1</sup></small> h<small><sup>−1</sup></small>).</p>\",\"PeriodicalId\":92,\"journal\":{\"name\":\"Nanoscale\",\"volume\":\" 6\",\"pages\":\" 3381-3388\"},\"PeriodicalIF\":5.8000,\"publicationDate\":\"2024-12-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanoscale\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/nr/d4nr02177k\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanoscale","FirstCategoryId":"88","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/nr/d4nr02177k","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
In vivo synthesis of semiconductor nanoparticles in Azotobacter vinelandii for light-driven ammonia production†
Ammonia (NH3) is an important commodity chemical used as an agricultural fertilizer and hydrogen-storage material. There has recently been much interest in developing an environmentally benign process for NH3 synthesis. Here, we report enhanced production of ammonia from diazotrophs under light irradiation using hybrid composites of inorganic nanoparticles (NPs) and bacterial cells. The primary focus of this study lies in the intracellular biosynthesis of semiconductor NPs within Azotobacter vinelandii, a diazotroph, when bacterial cells are cultured in a medium containing precursor molecules. For example, enzymes in bacterial cells, such as cysteine desulfurase, convert cysteine (Cys) into precursors for cadmium sulfide (CdS) synthesis when supplied with CdCl2. Photoexcited charge carriers in the biosynthesized NPs are transferred to nitrogen fixation enzymes, e.g., nitrogenase, facilitating the production of ammonium ions. Notably, the intracellular biosynthesis approach minimizes cell toxicity compared to extracellular synthesis due to the diminished generation of reactive oxygen species. The biohybrid system based on the in vivo approach results in a fivefold increase in ammonia production (0.45 mg gDCW−1 h−1) compared to the case of diazotroph cells only (0.09 mg gDCW−1 h−1).
期刊介绍:
Nanoscale is a high-impact international journal, publishing high-quality research across nanoscience and nanotechnology. Nanoscale publishes a full mix of research articles on experimental and theoretical work, including reviews, communications, and full papers.Highly interdisciplinary, this journal appeals to scientists, researchers and professionals interested in nanoscience and nanotechnology, quantum materials and quantum technology, including the areas of physics, chemistry, biology, medicine, materials, energy/environment, information technology, detection science, healthcare and drug discovery, and electronics.