巨噬细胞膜包被水凝胶微颗粒吸入体内减轻急性肺损伤的炎症作用。

IF 9.4 1区医学 Q1 ENGINEERING, BIOMEDICAL

Acta Biomaterialia Pub Date : 2025-01-15 DOI:10.1016/j.actbio.2024.12.015

Liang Song , Zihe Zhai , Wei Ouyang , Jie Ding , Shuqin Wang , Shifen Li , Min Liang , Feng Xu , Changyou Gao

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引用次数: 0

摘要

水凝胶微粒（HMPs）在生物医学应用方面具有许多优势，特别是在微创治疗方面，例如，急性肺损伤（ALI）的特点是微环境中含有高水平的活性氧（ROS）和促炎介质。本研究采用膜乳化装置设计并制备了具有ros清除和促炎细胞因子中和作用的HMPs。HMPs由双键修饰的透明质酸和含有硫酮键和不饱和双键的ros -可切割的超支化聚丙烯酸酯包硫酮-含乙二醇（HBPAK）组成。通过静电力对炎性巨噬细胞（M1）细胞膜进行表面包被，获得膜包被HBPAK HMPs （mem HMPs）。Mem HMPs在体外和体内均表现出较强的ros清除和抗炎特性。在ALI小鼠模型中吸入后，mem HMPs可减少中性粒细胞浸润和组织氧化损伤，从而减轻肺部炎症。我们的研究结果表明，mem HMPs可以作为一个潜在的治疗平台，有效地治疗炎症性疾病。意义声明：微创递送的水凝胶微粒（HMPs）有利于以高水平活性氧（ROS）和促炎介质为特征的急性肺损伤（ALI）。在微流控装置中，将双键修饰的透明质酸与含有硫酮键和不饱和双键的ros可切割的超支化聚（丙烯酸酯包覆的含硫酮-乙二醇）（HBPAK）共聚，制备了清除ros和促炎细胞因子中和的HMPs。被炎性巨噬细胞（M1）细胞膜覆盖的HMPs表现出强大的ros清除和抗炎特性，减少中性粒细胞浸润和组织氧化损伤，从而减轻肺部炎症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Inhalation of macrophage membrane-coated hydrogel microparticles for inflammation alleviation of acute lung injury in vivo

查看原文本刊更多论文

Inhalation of macrophage membrane-coated hydrogel microparticles for inflammation alleviation of acute lung injury in vivo

Hydrogel microparticles (HMPs) have many advantages for biomedical applications, particularly for minimally invasive therapy, for example, acute lung injury (ALI) that is characterized by high levels of reactive oxygen species (ROS) and pro-inflammatory mediators in the microenvironment. In this study, ROS-scavenging and pro-inflammatory cytokine-neutralizing HMPs were designed and prepared by using a membrane emulsification device. The HMPs were composed of double bond-modified hyaluronic acid and ROS-cleavable hyperbranched poly(acrylate-capped thioketone-containing ethylene glycol) (HBPAK) containing thioketal linkages and unsaturated double bonds. Surface-coating of inflammatory macrophage (M1) cell membranes was performed to obtain the membrane-coated HBPAK HMPs (mem HMPs) via electrostatic force. The mem HMPs exhibited strong ROS-scavenging and anti-inflammatory properties both in vitro and in vivo. After administered by inhalation in an ALI mouse model, the mem HMPs reduced neutrophil infiltration and tissue oxidative damage, thereby alleviating lung inflammation. Our results suggest that the mem HMPs could serve as a potential therapeutic platform for treating inflammatory diseases with high efficiency.

Statement of significance

Hydrogel microparticles (HMPs) with minimally invasive delivery are advantageous for acute lung injury (ALI) characterized by high levels of reactive oxygen species (ROS) and pro-inflammatory mediators. Herein, ROS-scavenging and pro-inflammatory cytokine-neutralizing HMPs were prepared by copolymerizing double bond-modified hyaluronic acid and ROS-cleavable hyperbranched poly(acrylate-capped thioketone-containing ethylene glycol) (HBPAK) containing thioketal bonds and unsaturated double bonds in a membrane emulsification device. The HMPs covered with inflammatory macrophage (M1) cell membranes (mem HMPs) exhibited strong ROS-scavenging and anti-inflammation properties, reduced neutrophil infiltration and tissue oxidative damage, thereby alleviating lung inflammation.

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来源期刊

Acta Biomaterialia 工程技术-材料科学：生物材料

CiteScore

16.80

自引率

3.10%

发文量

776

审稿时长

30 days

期刊介绍： Acta Biomaterialia is a monthly peer-reviewed scientific journal published by Elsevier. The journal was established in January 2005. The editor-in-chief is W.R. Wagner (University of Pittsburgh). The journal covers research in biomaterials science, including the interrelationship of biomaterial structure and function from macroscale to nanoscale. Topical coverage includes biomedical and biocompatible materials.