Danilo Alves de França, Sara Zúquete, Mariana Louro, Maíra Guimarães Kersul, Benedito Donizete Menozzi, Felipe Fornazari, Gabriela Santos-Gomes, Isabel Pereira da Fonseca, Helio Langoni
{"title":"巴西圣保罗州流行区和非流行区蝙蝠体内婴儿利什曼原虫和亚马逊利什曼原虫的检测。","authors":"Danilo Alves de França, Sara Zúquete, Mariana Louro, Maíra Guimarães Kersul, Benedito Donizete Menozzi, Felipe Fornazari, Gabriela Santos-Gomes, Isabel Pereira da Fonseca, Helio Langoni","doi":"10.1111/zph.13201","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aims</h3>\n \n <p>Leishmaniasis is an endemic disease in several regions of Brazil, a tropical country that presents specific environmental conditions that contribute to the development of phlebotomine vectors. This study aimed to detect <i>Leishmania</i> species in naturally infected bats from 17 municipalities in the São Paulo state.</p>\n </section>\n \n <section>\n \n <h3> Methods and Results</h3>\n \n <p>Spleen and liver samples from 203 bats were analysed by real-time PCR and confirmed by conventional PCR followed by gene sequencing. <i>Leishmania</i> DNA was amplified by real-time PCR in 6.4% of the bats and by conventional PCR followed by sequencing in 3.4% of the bats. Positive samples were characterised and included in GenBank. <i>Leishmania</i> species were confirmed in \n <i>M. molossus</i>\n , \n <i>M. nigricans</i>\n and \n <i>E. glaucinus</i>\n bats. <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> and <i>L. infantum</i> (syn. <i>L. chagasi</i>) were identified. This is the first detection of <i>Leishmania</i> spp. in bats in the studied areas. All the positive bats came from urban areas. Insectivorous bats were statistically more positive. There was similarity between our sequences and those of a human isolate and a phlebotomine from the region.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>This result points to bats as important possible reservoir of <i>Leishmania</i> in Brazil and guides the country's health authorities towards epidemiological surveillance, control and prevention actions in endemic areas.</p>\n </section>\n </div>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":"72 3","pages":"259-268"},"PeriodicalIF":2.4000,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of Leishmania infantum and Leishmania amazonensis in Bats From Endemic and Non-endemic Areas of São Paulo State, Brazil\",\"authors\":\"Danilo Alves de França, Sara Zúquete, Mariana Louro, Maíra Guimarães Kersul, Benedito Donizete Menozzi, Felipe Fornazari, Gabriela Santos-Gomes, Isabel Pereira da Fonseca, Helio Langoni\",\"doi\":\"10.1111/zph.13201\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Aims</h3>\\n \\n <p>Leishmaniasis is an endemic disease in several regions of Brazil, a tropical country that presents specific environmental conditions that contribute to the development of phlebotomine vectors. This study aimed to detect <i>Leishmania</i> species in naturally infected bats from 17 municipalities in the São Paulo state.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods and Results</h3>\\n \\n <p>Spleen and liver samples from 203 bats were analysed by real-time PCR and confirmed by conventional PCR followed by gene sequencing. <i>Leishmania</i> DNA was amplified by real-time PCR in 6.4% of the bats and by conventional PCR followed by sequencing in 3.4% of the bats. Positive samples were characterised and included in GenBank. <i>Leishmania</i> species were confirmed in \\n <i>M. molossus</i>\\n , \\n <i>M. nigricans</i>\\n and \\n <i>E. glaucinus</i>\\n bats. <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> and <i>L. infantum</i> (syn. <i>L. chagasi</i>) were identified. This is the first detection of <i>Leishmania</i> spp. in bats in the studied areas. All the positive bats came from urban areas. Insectivorous bats were statistically more positive. There was similarity between our sequences and those of a human isolate and a phlebotomine from the region.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>This result points to bats as important possible reservoir of <i>Leishmania</i> in Brazil and guides the country's health authorities towards epidemiological surveillance, control and prevention actions in endemic areas.</p>\\n </section>\\n </div>\",\"PeriodicalId\":24025,\"journal\":{\"name\":\"Zoonoses and Public Health\",\"volume\":\"72 3\",\"pages\":\"259-268\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2024-12-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zoonoses and Public Health\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/zph.13201\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zoonoses and Public Health","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/zph.13201","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Detection of Leishmania infantum and Leishmania amazonensis in Bats From Endemic and Non-endemic Areas of São Paulo State, Brazil
Aims
Leishmaniasis is an endemic disease in several regions of Brazil, a tropical country that presents specific environmental conditions that contribute to the development of phlebotomine vectors. This study aimed to detect Leishmania species in naturally infected bats from 17 municipalities in the São Paulo state.
Methods and Results
Spleen and liver samples from 203 bats were analysed by real-time PCR and confirmed by conventional PCR followed by gene sequencing. Leishmania DNA was amplified by real-time PCR in 6.4% of the bats and by conventional PCR followed by sequencing in 3.4% of the bats. Positive samples were characterised and included in GenBank. Leishmania species were confirmed in
M. molossus
,
M. nigricans
and
E. glaucinus
bats. Leishmania (Leishmania) amazonensis and L. infantum (syn. L. chagasi) were identified. This is the first detection of Leishmania spp. in bats in the studied areas. All the positive bats came from urban areas. Insectivorous bats were statistically more positive. There was similarity between our sequences and those of a human isolate and a phlebotomine from the region.
Conclusions
This result points to bats as important possible reservoir of Leishmania in Brazil and guides the country's health authorities towards epidemiological surveillance, control and prevention actions in endemic areas.
期刊介绍：
Zoonoses and Public Health brings together veterinary and human health researchers and policy-makers by providing a venue for publishing integrated and global approaches to zoonoses and public health. The Editors will consider papers that focus on timely collaborative and multi-disciplinary research in zoonoses and public health. This journal provides rapid publication of original papers, reviews, and potential discussion papers embracing this collaborative spirit. Papers should advance the scientific knowledge of the sources, transmission, prevention and control of zoonoses and be authored by scientists with expertise in areas such as microbiology, virology, parasitology and epidemiology. Articles that incorporate recent data into new methods, applications, or approaches (e.g. statistical modeling) which enhance public health are strongly encouraged.
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