基于免疫分析法的高特异性单克隆抗体检测SARS-CoV-2生物传感器平台设计

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-12-03 DOI:10.1016/j.diagmicrobio.2024.116644

Göknur Gizem Dinç , Ebru Saatçi , İlkay Göksu Polat , Fatıma Yücel , Uygar Halis Tazebay , Esin Akçael

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引用次数: 0

摘要

SARS-CoV-2在全球的蔓延凸显了早期和快速发现对控制大流行传播的重要性。在本研究中，针对病毒的保守核衣壳蛋白，在不同的变体中制备了特异性和高亲和力的单克隆抗体（mab）。选择合适的抗体对开发横向流动免疫测定（LFIA）和使用未经修饰的丝网印刷电极和外部磁珠制备的安培生物传感器的非常规应用。在本研究中，我们开发的LFIA检测SARS-CoV-2病毒的LOD为104 PFU/mL，而安培生物传感器检测灭活SARS-CoV-2的LOD为5.5 PFU/mL。经过对系统的验证，我们获得的单克隆抗体将能够在LFIA和安培免疫传感器平台上灵敏和选择性地检测SARS-CoV-2，用于临床诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Design of immunoassay based biosensor platforms for SARS-CoV-2 detection using highly specific monoclonal antibodies

查看原文本刊更多论文

Design of immunoassay based biosensor platforms for SARS-CoV-2 detection using highly specific monoclonal antibodies

The global expand of SARS-CoV-2 has highlighted the importance of early and rapid detection to control the spread of a pandemic. In this study, specific and high-affinity monoclonal antibodies (mAbs) were developed against the conserved nucleocapsid protein of the virus among variants. Appropriate antibody pairs were selected to develop a lateral flow immunoassay (LFIA) and an unconventional application of an amperometric biosensor using unmodified screen-printed electrodes and external magnetic bead preparation. In the study, the LFIA we developed detected the SARS-CoV-2 virus at 10⁴ PFU/mL, while the amperometric biosensor enabled sensitive detection of inactivated SARS-CoV-2 with an LOD of 5.5 PFU/mL. After validating the developed systems, it is considered that the mAbs we have obtained will enable the sensitive and selective detection of SARS-CoV-2 in LFIA and amperometric immunosensor platforms for clinical diagnosis.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.