Maternal supplementation with Dipteryx alata Vog. modulates fecal microbiota diversity, accelerates reflex ontogeny, and improves non-associative and spatial memory in the offspring of rats.

Maternal diet plays a crucial role in offspring development, directly affecting neural development and gut microbiota composition. This study aimed to assess if baru almond and oil (Dipteryx alata Vog.) could modulate intestinal microbiota, brain fatty acid profile, and enhance memory in offspring of rats treated during early life stages. Three groups were formed: Control- received distilled water by gavage; Oil- received 2000 mg/kg of baru oil, and Almond - received 2000 mg/kg of baru almond. Somatic development and reflex ontogenesis were evaluated in offspring during the first 21 days. In adolescence and adulthood, memory was tested using Open Field Habituation, Object Recognition, and Morris Water Maze. Brain histology and fatty acid were measured, and fecal microbiota analysis was performed. Both almond and oil groups showed increased PUFAs in breast milk and brains, accelerated reflex ontogeny, improved somatic development and better performance in the memory tests in both life stages (p < 0.05). Supplementation enhanced fecal microbiota abundance associated with neuroprotective effects. The almond group showed a 29 % increase in Eubacterium, Candidates-Arthromitus, Collinsella, and Christensenellaceae-R-7. Both oil and almond groups had higher Blautia and Clostridia-UCG-014 compared to controls. The oil group had about 10 % more Ruminococcus, UCG-005, Acetatifactor, Negativibacillus, and Lachnospiraceae-ND3007 than the others. With the present data, we can observe the safety of baru consumption by pregnant and lactating rats and verify its effects on modulating the microbiota, inducing adequate development of the offspring's nervous system, contributing to anticipated reflex maturation and improving memory.