解码草药和膳食补充剂治疗绝经后骨质疏松症的协同潜力。

IF 3.9

Experimental gerontology Pub Date : 2025-01-01 DOI:10.1016/j.exger.2024.112648

Jinfang Dou , Xuehong Hu , Junchang Liu , Jiaxian Liu , Chuyue Zhang , Yuelin Zhang , Xianjie Zhen , Siyu Tao , Jinghua Zhang , Guangjian Jiang

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引用次数: 0

摘要

目的：探索藜麦与FLL的联合作用，为提高骨质疏松症的疗效提供了一个有趣的机会。方法：利用网络药理学方法预测藜麦和藜麦的潜在抗骨质疏松作用，并通过实验验证。30只大鼠分为5组：假手术组（SO）、去卵巢组（OVX）、FLL组（FLL）、藜麦组（quinoa）、FLL联合藜麦组（F&Q）。为期12周的实验包括骨密度和微结构测量、股骨小梁和骨皮质组织学评估、股骨破骨细胞定量、破骨细胞分化因子表达（NFATc1和C-Fos）评估、I型胶原羧基末端肽（CTX-1）测量。此外，进行肠道菌群分析。结果：OVX组骨密度降低，骨微结构受损，破骨细胞数量和分化增加，骨吸收增强，FLL、藜麦和F&Q干预完全改善了这一现象。与FLL和藜麦相比，F&Q在骨密度和微结构方面表现出更好的改善，尽管它们对破骨细胞和骨吸收的影响没有显著差异。肠道菌群分析显示，F&Q在缓解ovx诱导的肠道菌群紊乱方面比F&Q和藜麦更有效，特别是在增强肠道菌群多样性和功能方面。结论：藜麦与黄芪多糖联合治疗卵巢切除所致骨质疏松及肠道菌群失调的疗效优于单用黄芪多糖。

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Decoding the synergistic potential of herbal medicine and dietary supplements for treating postmenopausal osteoporosis

Purpose

The exploration of the combined effects of FLL and quinoa presents an intriguing opportunity to enhance the efficacy of osteoporosis treatment.

Methods

This study aimed to predict the potential anti-osteoporotic effects of FLL and quinoa using network pharmacology and subsequently verify these predictions through experimental investigation. Thirty rats were divided into five groups: sham operation group (SO), ovariectomized group (OVX), FLL group (FLL), quinoa group (Quinoa), and FLL combined with quinoa group (F&Q). The 12-week experiment involved measurements of bone density and microstructure, histological evaluation of femoral trabeculae and bone cortex, quantification of osteoclasts in the femur, assessment of osteoclast differentiation factor expression (NFATc1 and C-Fos), and measurement of collagen I carboxyl-terminal peptide (CTX-1). Additionally, intestinal flora analysis was performed.

Results

The results demonstrated reduced bone mineral density, compromised bone microarchitecture, increased osteoclast numbers and differentiation, and enhanced bone resorption in the OVX group, which were completely ameliorated by FLL, quinoa and F&Q interventions. F&Q exhibited superior improvement in bone density and microarchitecture compared to FLL and quinoa, although no significant differences were observed in their effects on osteoclasts and bone resorption. Gut flora analysis revealed that F&Q was more effective than FLL and quinoa in alleviating OVX-induced intestinal flora disorders, particularly in terms of enhancing intestinal flora diversity and function.