OsbZIP27 coordinates with OsHUB1 and OsHUB2 to modulate drought tolerance in rice.

Histone H2B ubiquitination (H2Bub) is positively linked to transcriptional activation, but the genetic programs affected by H2Bub to enhance drought tolerance remain largely unknown. Here, we show that OsbZIP27 interacts directly with OsHUB1/2 to regulate drought tolerance in rice by binding to the promoters of OsHAK1 and OsGLN1 to achieve H2Bub and transcriptional activation. Consistently, mutations in OsbZIP27 reduce transcription of OsHAK1 and OsGLN1, resulting in increased sensitivity to drought stress. Moreover, loss of OsHUB1 and OsHUB2 function causes hypersensitivity to drought stress, whereas OsHUB2 overexpression enhances drought tolerance. Together, our results indicate that OsbZIP27 coordinates with OsHUB1/2 to enhance rice drought tolerance by increasing H2Bub and expression of OsHAK1 and OsGLN1.