基因敲除LINC01405的人胚胎干细胞系的产生与表征

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-02-01 Epub Date: 2024-12-01 DOI:10.1016/j.scr.2024.103619

Yaping Xu, Hongchun Wu, Jingxiu Jiang, Lingqun Ye, Kaili Hao, Kunjun Han, Shijun Hu, Wei Lei, Zhikun Guo

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引用次数: 0

摘要

长基因间非蛋白编码RNA 1405 (LINC01405)，已知在肌肉中表达升高，与许多肌肉骨骼疾病有关。利用CRISPR/Cas9基因编辑系统，我们构建了一个敲除LINC01405的人胚胎干细胞（hESC）细胞系。该细胞系保持了人类干细胞样的形态和多能性，表现出正常的核型，并能从所有三个胚层分化成细胞。该细胞系将成为研究LINC01405在心脏和骨骼肌正常发育及其疾病中的作用的宝贵模型。

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Generation and characterization of the LINC01405 knockout human embryonic stem cell line.

Long Intergenic Non-Protein Coding RNA 1405 (LINC01405), with known elevated expression in muscle, has been linked to a number of musculo-skeletal conditions. By utilizing the CRISPR/Cas9 gene editing system, we generated a LINC01405 knockout human embryonic stem cell (hESC) line. This line remains human stem cell-like morphology and pluripotency, exhibits a normal karyotype, and can differentiate into cells from all three germ layers. This cell line will be an invaluable model for the research on LINC01405's role in normal development of cardiac and skeletal muscle, and their diseases.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.