Th1/Th2和Tc1/Tc2失衡与婴儿肝移植受者巨细胞病毒活动性感染相关

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings Pub Date : 2024-12-01 DOI:10.1016/j.transproceed.2024.10.036

Dan Li , Chun Liu , Zhongyu Kang , Yan Zheng , Yuliang Wang

{"title":"Th1/Th2和Tc1/Tc2失衡与婴儿肝移植受者巨细胞病毒活动性感染相关","authors":"Dan Li , Chun Liu , Zhongyu Kang , Yan Zheng , Yuliang Wang","doi":"10.1016/j.transproceed.2024.10.036","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Because cytomegalovirus (CMV) infection is one of the most common complications following liver transplantation (LT), it is important to analyze the impact of CMV infection on the LT-associated changes in T cells polarization. This study aimed to investigate T helper (Th) and T cytotoxic (Tc) cells polarization and their correlation in infant LT recipients with active CMV infection.</div></div><div><h3>Methods</h3><div>Twenty infant LT recipients with active CMV infection (the CMV group) and 20 recipients without CMV infection (the stable group) were enrolled. The percentages of Th1, Th2, Tc1, and Tc2 cells were detected by flow cytometry after intracellular staining for cytokines (IFN-γ and IL-10, respectively) in peripheral blood. The correlation between Th and Tc cells was analyzed by Pearson correlation coefficient.</div></div><div><h3>Results</h3><div>The percentages of Th1 and Tc1 cells were significantly decreased, whereas the percentages of Tc2 cells were significantly increased in CMV group compared with the stable group, along with significant reduction of Th1/Th2 and Tc1/Tc2 ratios (<em>P</em> < .01). The percentages of Th1 cells were positively correlated with Tc1 cells (<em>P</em> < .01). A higher Th1/Th2 and Tc1/Tc2 ratios were showed in the CMV group after antiviral therapy than those in the CMV group before therapy (<em>P</em> < .01).</div></div><div><h3>Conclusions</h3><div>Our findings show an imbalanced Th1/Th2 and Tc1/Tc2 immunity in infant LT recipients with active CMV infection, which were involved in the pathogenesis of CMV infection.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"56 10","pages":"Pages 2172-2177"},"PeriodicalIF":0.8000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Imbalances of Th1/Th2 and Tc1/Tc2 are Associated With Active Cytomegalovirus Infection in Infant Liver Transplant Recipients\",\"authors\":\"Dan Li , Chun Liu , Zhongyu Kang , Yan Zheng , Yuliang Wang\",\"doi\":\"10.1016/j.transproceed.2024.10.036\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Because cytomegalovirus (CMV) infection is one of the most common complications following liver transplantation (LT), it is important to analyze the impact of CMV infection on the LT-associated changes in T cells polarization. This study aimed to investigate T helper (Th) and T cytotoxic (Tc) cells polarization and their correlation in infant LT recipients with active CMV infection.</div></div><div><h3>Methods</h3><div>Twenty infant LT recipients with active CMV infection (the CMV group) and 20 recipients without CMV infection (the stable group) were enrolled. The percentages of Th1, Th2, Tc1, and Tc2 cells were detected by flow cytometry after intracellular staining for cytokines (IFN-γ and IL-10, respectively) in peripheral blood. The correlation between Th and Tc cells was analyzed by Pearson correlation coefficient.</div></div><div><h3>Results</h3><div>The percentages of Th1 and Tc1 cells were significantly decreased, whereas the percentages of Tc2 cells were significantly increased in CMV group compared with the stable group, along with significant reduction of Th1/Th2 and Tc1/Tc2 ratios (<em>P</em> < .01). The percentages of Th1 cells were positively correlated with Tc1 cells (<em>P</em> < .01). A higher Th1/Th2 and Tc1/Tc2 ratios were showed in the CMV group after antiviral therapy than those in the CMV group before therapy (<em>P</em> < .01).</div></div><div><h3>Conclusions</h3><div>Our findings show an imbalanced Th1/Th2 and Tc1/Tc2 immunity in infant LT recipients with active CMV infection, which were involved in the pathogenesis of CMV infection.</div></div>\",\"PeriodicalId\":23246,\"journal\":{\"name\":\"Transplantation proceedings\",\"volume\":\"56 10\",\"pages\":\"Pages 2172-2177\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Transplantation proceedings\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0041134524005815\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transplantation proceedings","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0041134524005815","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

背景：巨细胞病毒（CMV）感染是肝移植（LT）术后最常见的并发症之一，分析巨细胞病毒感染对LT相关T细胞极化变化的影响具有重要意义。本研究旨在探讨辅助性T细胞（Th）和T细胞毒性（Tc）细胞极化及其在巨细胞病毒活动性感染的婴儿LT受体中的相关性。方法：选取20例伴有巨细胞病毒活动性感染的婴儿肝移植受体（CMV组）和20例无巨细胞病毒感染的婴儿肝移植受体（稳定组）。细胞内细胞因子（分别为IFN-γ和IL-10）染色后，流式细胞术检测外周血中Th1、Th2、Tc1和Tc2细胞的百分比。采用Pearson相关系数分析Th细胞与Tc细胞的相关性。结果：CMV组与稳定组比较，Th1、Tc1细胞百分比显著降低，Tc2细胞百分比显著升高，Th1/Th2、Tc1/Tc2比值显著降低（P < 0.01）。Th1细胞百分比与Tc1细胞百分比呈正相关（P < 0.01）。抗病毒治疗后CMV组Th1/Th2、Tc1/Tc2比值明显高于治疗前CMV组（P < 0.01）。结论：我们的研究结果表明，活动性巨细胞病毒感染的婴儿LT受体中Th1/Th2和Tc1/Tc2免疫失衡，这参与了巨细胞病毒感染的发病机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Imbalances of Th1/Th2 and Tc1/Tc2 are Associated With Active Cytomegalovirus Infection in Infant Liver Transplant Recipients

Background

Because cytomegalovirus (CMV) infection is one of the most common complications following liver transplantation (LT), it is important to analyze the impact of CMV infection on the LT-associated changes in T cells polarization. This study aimed to investigate T helper (Th) and T cytotoxic (Tc) cells polarization and their correlation in infant LT recipients with active CMV infection.

Methods

Twenty infant LT recipients with active CMV infection (the CMV group) and 20 recipients without CMV infection (the stable group) were enrolled. The percentages of Th1, Th2, Tc1, and Tc2 cells were detected by flow cytometry after intracellular staining for cytokines (IFN-γ and IL-10, respectively) in peripheral blood. The correlation between Th and Tc cells was analyzed by Pearson correlation coefficient.

Results

The percentages of Th1 and Tc1 cells were significantly decreased, whereas the percentages of Tc2 cells were significantly increased in CMV group compared with the stable group, along with significant reduction of Th1/Th2 and Tc1/Tc2 ratios (P < .01). The percentages of Th1 cells were positively correlated with Tc1 cells (P < .01). A higher Th1/Th2 and Tc1/Tc2 ratios were showed in the CMV group after antiviral therapy than those in the CMV group before therapy (P < .01).

Conclusions

Our findings show an imbalanced Th1/Th2 and Tc1/Tc2 immunity in infant LT recipients with active CMV infection, which were involved in the pathogenesis of CMV infection.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Transplantation proceedings 医学-免疫学

CiteScore

1.70

自引率

0.00%

发文量

502

审稿时长

60 days

期刊介绍： Transplantation Proceedings publishes several different categories of manuscripts, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. The first type of manuscripts consists of sets of papers providing an in-depth expression of the current state of the art in various rapidly developing components of world transplantation biology and medicine. These manuscripts emanate from congresses of the affiliated transplantation societies, from Symposia sponsored by the Societies, as well as special Conferences and Workshops covering related topics. Transplantation Proceedings also publishes several special sections including publication of Clinical Transplantation Proceedings, being rapid original contributions of preclinical and clinical experiences. These manuscripts undergo review by members of the Editorial Board. Original basic or clinical science articles, clinical trials and case studies can be submitted to the journal?s open access companion title Transplantation Reports.