Ameliorative role of Tetrapleura tetraptera (Schum. & Thonn.) taub in cadmium chloride-induced oxidative stress in Drosophila melanogaster using in vivo and computational approaches.

Background: Cadmium (Cd) is a naturally occurring transition metal associated with oxidative stress in living organisms. Whereas Tetrapleura tetraptera (Tt), an ethnomedicinal plant is said to possess high antioxidant activity and used to treat various human diseases locally. Therefore, the study aimed to investigate the biological activity of the ethanolic pod extract of T. tetraptera in cadmium chloride-induced toxicity in Drosophila melanogaster.

Methods: Six groups of adults (1-3 days old) D. melanogaster as shown: Control, Tt 2.5 mg/10 g diet, Tt 5 mg/10 g diet, CdCl₂, CdCl₂+ Tt 2.5 mg/10 diet and CdCl₂+Tt 5 mg/10 g diet were exposed via diet for 7 days consisting of 50 flies per vial and 5 replicate per group. Thereafter, we evaluated markers for free radical generation, antioxidant, non-antioxidant activities, and emergence rates of the flies. The active compounds of Tt extract were molecularly docked against glutathione-S-transferase II.

Results: The results indicated that CdCl₂ significantly induced oxidative stress by increasing the levels of lipid peroxidation (LPO), hydrogen peroxide (H₂O₂)_, nitric oxide (NO) and decreasing the activity of GST without an effect on total thiol (T-SH) and non-protein thiols (NP-SHs) levels. However, co-treatment with T. tetraptera (2.5 mg/10 g diet) significantly decreased levels of LPO, H₂O_2, but increased GST activity. Also, co-treatment with T. tetraptera (5 mg/10 g diet) increased NPSH and T-SH levels by 18.6 % and 35.8 %. Furthermore, Co-treatment (5 mg/10 g diet) increased the rate of offspring emergence.

Conclusion: T. tetraptera ameliorated cadmium chloride-induced oxidative stress in Drosophila melanogaster and increased offspring hatching rate. T. tetraptera may therefore serve as a good regimen for the treatment of oxidative stress-related diseases induced by cadmium.