Xin Ru Zhou, Can Zhang, Chen Rong Xu, Xin Er Tan, Qian Qian Han, Xi Yang, Tian Yu Sun, Long Quan Shao, Jia Liu
{"title":"SIRT2介导的微管乙酰化在成骨分化中的作用","authors":"Xin Ru Zhou, Can Zhang, Chen Rong Xu, Xin Er Tan, Qian Qian Han, Xi Yang, Tian Yu Sun, Long Quan Shao, Jia Liu","doi":"10.3290/j.cjdr.b5860280","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To assess the role of microtubule acetylation in the transportation of amorphous calcium phosphate (ACP)-containing vesicles that mediate the osteogenic differentiation process of rat bone mesenchymal stem cells (BMSCs).</p><p><strong>Methods: </strong>Rat BMSCs were cultured and transfected with sirtuin 2 (SIRT2) overexpression plasmids for an in vitro model. The microtubule acetylation-related protein levels were detected by western blots. The microtubule acetylation and the secretion rate of extracellular ACPcontaining vesicles were observed with immunofluorescence and live cell fluorescence imaging. The secretion of ACP was observed by transmission electron microscopy. The mineralised nodule formation was stained with Alizarin Red S staining and observed by microscopy.</p><p><strong>Results: </strong>Microtubule acetylation was increased during osteogenic differentiation of BMSCs, and microtubule transport efficiency was enhanced. Mechanically, microtubule acetylation is the key reason for the increased transportation rate of ACP-containing vesicles and enhanced osteogenic differentiation, as both were blocked after SIRT2-mediated microtubule acetylation inhibition.</p><p><strong>Conclusion: </strong>Microtubule acetylation mainly promotes the transportation and secretion of ACP vesicles, and ultimately promotes the osteogenic differentiation process.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"27 4","pages":"303-310"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"SIRT2 Mediated Microtubule Acetylation in Osteogenic Differentiation.\",\"authors\":\"Xin Ru Zhou, Can Zhang, Chen Rong Xu, Xin Er Tan, Qian Qian Han, Xi Yang, Tian Yu Sun, Long Quan Shao, Jia Liu\",\"doi\":\"10.3290/j.cjdr.b5860280\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To assess the role of microtubule acetylation in the transportation of amorphous calcium phosphate (ACP)-containing vesicles that mediate the osteogenic differentiation process of rat bone mesenchymal stem cells (BMSCs).</p><p><strong>Methods: </strong>Rat BMSCs were cultured and transfected with sirtuin 2 (SIRT2) overexpression plasmids for an in vitro model. The microtubule acetylation-related protein levels were detected by western blots. The microtubule acetylation and the secretion rate of extracellular ACPcontaining vesicles were observed with immunofluorescence and live cell fluorescence imaging. The secretion of ACP was observed by transmission electron microscopy. The mineralised nodule formation was stained with Alizarin Red S staining and observed by microscopy.</p><p><strong>Results: </strong>Microtubule acetylation was increased during osteogenic differentiation of BMSCs, and microtubule transport efficiency was enhanced. Mechanically, microtubule acetylation is the key reason for the increased transportation rate of ACP-containing vesicles and enhanced osteogenic differentiation, as both were blocked after SIRT2-mediated microtubule acetylation inhibition.</p><p><strong>Conclusion: </strong>Microtubule acetylation mainly promotes the transportation and secretion of ACP vesicles, and ultimately promotes the osteogenic differentiation process.</p>\",\"PeriodicalId\":74983,\"journal\":{\"name\":\"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)\",\"volume\":\"27 4\",\"pages\":\"303-310\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-12-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3290/j.cjdr.b5860280\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3290/j.cjdr.b5860280","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
SIRT2 Mediated Microtubule Acetylation in Osteogenic Differentiation.
Objective: To assess the role of microtubule acetylation in the transportation of amorphous calcium phosphate (ACP)-containing vesicles that mediate the osteogenic differentiation process of rat bone mesenchymal stem cells (BMSCs).
Methods: Rat BMSCs were cultured and transfected with sirtuin 2 (SIRT2) overexpression plasmids for an in vitro model. The microtubule acetylation-related protein levels were detected by western blots. The microtubule acetylation and the secretion rate of extracellular ACPcontaining vesicles were observed with immunofluorescence and live cell fluorescence imaging. The secretion of ACP was observed by transmission electron microscopy. The mineralised nodule formation was stained with Alizarin Red S staining and observed by microscopy.
Results: Microtubule acetylation was increased during osteogenic differentiation of BMSCs, and microtubule transport efficiency was enhanced. Mechanically, microtubule acetylation is the key reason for the increased transportation rate of ACP-containing vesicles and enhanced osteogenic differentiation, as both were blocked after SIRT2-mediated microtubule acetylation inhibition.
Conclusion: Microtubule acetylation mainly promotes the transportation and secretion of ACP vesicles, and ultimately promotes the osteogenic differentiation process.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
