W A Pamudi Maldam Dewasmika, D R Anuruddhika Dissanayake, N G Dulanjalee Anuruddhika Kumari Neelawala, B G Nilanthi Dissanayake, D V Pahan Prasada, Veithehi Rajeevan Francis, Sophie Octavia, Ruiting Lan
{"title":"斯里兰卡人类和狗肠道外致病性大肠杆菌中ST131的高发生率","authors":"W A Pamudi Maldam Dewasmika, D R Anuruddhika Dissanayake, N G Dulanjalee Anuruddhika Kumari Neelawala, B G Nilanthi Dissanayake, D V Pahan Prasada, Veithehi Rajeevan Francis, Sophie Octavia, Ruiting Lan","doi":"10.1111/zph.13199","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims: </strong>Extraintestinal pathogenic Escherichia coli (ExPEC) is an important human and animal pathogen. In particular, the ST131 of ExPEC is a widely distributed clone, resistant to extended-spectrum cephalosporins and fluoroquinolones. We investigated the occurrence of ST131 among ExPEC from humans and dogs in Sri Lanka and determined its antimicrobial resistance.</p><p><strong>Methods: </strong>A total of 215 ExPEC isolates were collected from humans (n = 179) and dogs (n = 36) from two different cities in Sri Lanka. Antimicrobial resistance was tested by the disk diffusion method, and the presence of resistance-encoding genes (bla<sub>TEM</sub>, bla<sub>SHV</sub> and bla<sub>CTX-M</sub>) and ST131 clades/subclades was tested by PCR.</p><p><strong>Results: </strong>The majority of the isolates were (61.8%) ST131, with 55.8% in ST131-clade C. Of the clade C isolates, 44.3%, 27.8%, 15.7% and 6.7% were in clades C2, C other, C1-non-M27 and C1-M27, respectively. Approximately 11% of the ST131 isolates were carbapenem resistant. ExPEC from dogs showed comparable resistance rates to human isolates except for resistance to amoxicillin-clavulanate and amikacin.</p><p><strong>Conclusion: </strong>We observed a high occurrence of ST131 and its clade C, with more than 11% exhibiting resistance to carbapenems in Sri Lanka. Furthermore, ST131-C1-M27, with high resistance to both quinolones and extended-spectrum cephalosporins, was also present. Our results emphasise the importance of the One Health approach in the monitoring of antimicrobial-resistant E. coli in different regions of Sri Lanka to gain a better understanding of their prevalence over time, contributing to effective antimicrobial stewardship.</p>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":" ","pages":"200-206"},"PeriodicalIF":2.4000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"High Occurrence of ST131 Among Extraintestinal Pathogenic Escherichia coli in Humans and Dogs in Sri Lanka.\",\"authors\":\"W A Pamudi Maldam Dewasmika, D R Anuruddhika Dissanayake, N G Dulanjalee Anuruddhika Kumari Neelawala, B G Nilanthi Dissanayake, D V Pahan Prasada, Veithehi Rajeevan Francis, Sophie Octavia, Ruiting Lan\",\"doi\":\"10.1111/zph.13199\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>Extraintestinal pathogenic Escherichia coli (ExPEC) is an important human and animal pathogen. In particular, the ST131 of ExPEC is a widely distributed clone, resistant to extended-spectrum cephalosporins and fluoroquinolones. We investigated the occurrence of ST131 among ExPEC from humans and dogs in Sri Lanka and determined its antimicrobial resistance.</p><p><strong>Methods: </strong>A total of 215 ExPEC isolates were collected from humans (n = 179) and dogs (n = 36) from two different cities in Sri Lanka. Antimicrobial resistance was tested by the disk diffusion method, and the presence of resistance-encoding genes (bla<sub>TEM</sub>, bla<sub>SHV</sub> and bla<sub>CTX-M</sub>) and ST131 clades/subclades was tested by PCR.</p><p><strong>Results: </strong>The majority of the isolates were (61.8%) ST131, with 55.8% in ST131-clade C. Of the clade C isolates, 44.3%, 27.8%, 15.7% and 6.7% were in clades C2, C other, C1-non-M27 and C1-M27, respectively. Approximately 11% of the ST131 isolates were carbapenem resistant. ExPEC from dogs showed comparable resistance rates to human isolates except for resistance to amoxicillin-clavulanate and amikacin.</p><p><strong>Conclusion: </strong>We observed a high occurrence of ST131 and its clade C, with more than 11% exhibiting resistance to carbapenems in Sri Lanka. Furthermore, ST131-C1-M27, with high resistance to both quinolones and extended-spectrum cephalosporins, was also present. Our results emphasise the importance of the One Health approach in the monitoring of antimicrobial-resistant E. coli in different regions of Sri Lanka to gain a better understanding of their prevalence over time, contributing to effective antimicrobial stewardship.</p>\",\"PeriodicalId\":24025,\"journal\":{\"name\":\"Zoonoses and Public Health\",\"volume\":\" \",\"pages\":\"200-206\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zoonoses and Public Health\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1111/zph.13199\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/12/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zoonoses and Public Health","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/zph.13199","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/5 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
High Occurrence of ST131 Among Extraintestinal Pathogenic Escherichia coli in Humans and Dogs in Sri Lanka.
Aims: Extraintestinal pathogenic Escherichia coli (ExPEC) is an important human and animal pathogen. In particular, the ST131 of ExPEC is a widely distributed clone, resistant to extended-spectrum cephalosporins and fluoroquinolones. We investigated the occurrence of ST131 among ExPEC from humans and dogs in Sri Lanka and determined its antimicrobial resistance.
Methods: A total of 215 ExPEC isolates were collected from humans (n = 179) and dogs (n = 36) from two different cities in Sri Lanka. Antimicrobial resistance was tested by the disk diffusion method, and the presence of resistance-encoding genes (blaTEM, blaSHV and blaCTX-M) and ST131 clades/subclades was tested by PCR.
Results: The majority of the isolates were (61.8%) ST131, with 55.8% in ST131-clade C. Of the clade C isolates, 44.3%, 27.8%, 15.7% and 6.7% were in clades C2, C other, C1-non-M27 and C1-M27, respectively. Approximately 11% of the ST131 isolates were carbapenem resistant. ExPEC from dogs showed comparable resistance rates to human isolates except for resistance to amoxicillin-clavulanate and amikacin.
Conclusion: We observed a high occurrence of ST131 and its clade C, with more than 11% exhibiting resistance to carbapenems in Sri Lanka. Furthermore, ST131-C1-M27, with high resistance to both quinolones and extended-spectrum cephalosporins, was also present. Our results emphasise the importance of the One Health approach in the monitoring of antimicrobial-resistant E. coli in different regions of Sri Lanka to gain a better understanding of their prevalence over time, contributing to effective antimicrobial stewardship.
期刊介绍：
Zoonoses and Public Health brings together veterinary and human health researchers and policy-makers by providing a venue for publishing integrated and global approaches to zoonoses and public health. The Editors will consider papers that focus on timely collaborative and multi-disciplinary research in zoonoses and public health. This journal provides rapid publication of original papers, reviews, and potential discussion papers embracing this collaborative spirit. Papers should advance the scientific knowledge of the sources, transmission, prevention and control of zoonoses and be authored by scientists with expertise in areas such as microbiology, virology, parasitology and epidemiology. Articles that incorporate recent data into new methods, applications, or approaches (e.g. statistical modeling) which enhance public health are strongly encouraged.
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