培养利什曼原虫提取和纯化脂磷聚糖的方法。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-12-04 DOI:10.1016/j.xpro.2024.103468

Lisa Ulrike Teufel, Jéssica Cristina Dos Santos

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引用次数: 0

摘要

脂磷聚糖（LPG）是利什曼原虫表面的一种大分子。在寄生虫的整个生命周期中，LPG的生化结构在增殖（前循环）和感染（元循环）阶段以及物种和菌株之间都是不同的。在这里，我们概述了体外培养利什曼原虫以收获LPG的方案。我们描述了寄生虫分化和液化石油气提取和纯化的步骤。液化石油气在医学研究中有应用，例如在训练免疫和癌症免疫治疗中。有关该协议的使用和执行的完整细节，请参阅dos Santos等人。

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Protocol to culture Leishmania protozoans for lipophosphoglycan extraction and purification.

Lipophosphoglycan (LPG) is a macromolecule on the surface of Leishmania spp. parasites. The biochemical structure of LPG varies throughout the parasites' life cycle between proliferative (procyclic) and infective (metacyclic) stages, as well as between species and strains. Here, we outline a protocol for growing Leishmania parasites in vitro to harvest LPG. We describe steps for parasite differentiation and LPG extraction and purification. LPG has applications in medical research, such as in trained immunity and immunotherapy for cancer. For complete details on the use and execution of this protocol, please refer to dos Santos et al.¹.

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