探索他腊杉醇对小鼠的抗伤害感受作用：可能的机制。

IF 2.5 4区医学 Q3 NEUROSCIENCES

Neuroscience Letters Pub Date : 2025-01-10 DOI:10.1016/j.neulet.2024.138075

Cagil Onal Sis , Yagmur Okcay , Kemal Gokhan Ulusoy , Ismail Mert Vural , Oguzhan Yıldız

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引用次数: 0

摘要

目的：taraxastrol是蒲公英的有效成分，因其具有多种治疗作用而在传统医学中得到广泛应用。本研究旨在首先评估taraxasterol潜在的脊髓/椎管上和外周/内脏抗伤性作用，然后研究gaba能、阿片能系统和KATP通道对其抗伤性作用的贡献。方法：采用热板法、尾浸法和醋酸致腹部扭体法（分别用于椎上、脊柱、外周/内脏疼痛评估）对BALB/c雄性小鼠（2.5、5和10 mg/kg i.p）进行抗伤性实验，并计算可能的最大效应百分比（MPE%）值。作用机制研究是通过预先给药双库兰、纳洛酮和格列本脲进行的。结果：在2.5、5和10 mg/kg剂量的热板和尾浸试验中，他乐松醇增加了MPE%值（P ）。结论：我们的研究首次表明，他乐松醇在椎骨上和脊柱疼痛试验中的抗痛觉作用涉及gaba能和阿片能系统，以及在评估椎骨上、脊柱和外周疼痛通路的试验中，他乐松醇参与了KATP通道。塔拉松醇是一种潜在的新型草药，可用于控制疼痛。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Exploring the antinociceptive effect of taraxasterol in mice: Possible mechanisms

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Exploring the antinociceptive effect of taraxasterol in mice: Possible mechanisms

Objectives

Taraxasterol is the active ingredient of Taraxacum officinale which has been used in traditional medicine for its several therapeutic effects. This study aims first to evaluate the potential spinal/supraspinal and peripheral/visceral antinociceptive effect of taraxasterol and then to investigate the contribution of GABAergic, opioidergic systems, and K_ATP channels to its antinociceptive effect.

Methods

The antinociceptive activity of taraxasterol (2.5, 5, and 10 mg/kg i.p.) was investigated with hot-plate, tail-immersion, and acetic acid-induced abdominal writhing tests (for supraspinal, spinal, peripheral/visceral pain evaluation, respectively) in BALB/c male mice, and percentage of possible maximum effect (MPE%) values were calculated. Mechanism of action studies were performed by pre-administering bicuculline, naloxone, and glibenclamide.

Results

Taraxasterol increased the MPE% values in hot-plate and tail-immersion tests at 2.5, 5, and 10 mg/kg doses (P < 0.001) and decreased the mean number of writhes at 10 mg/kg in the abdominal writhing test (P < 0.05). Naloxone and bicuculline pre-administration reversed the antinociceptive effect of taraxasterol in hot-plate and tail-immersion tests and it had no effect in the abdominal writhing test. Pre-administration of glibenclamide reversed the antinociceptive effect of taraxasterol in all tests.

Conclusion

Our study is the first to show the involvement of GABAergic and opioidergic systems in the antinociceptive effect of taraxasterol in supraspinal and spinal pain tests, and K_ATP channels in tests evaluating supraspinal, spinal, and peripheral pain pathways. Taraxasterol is a potential new herbal medicine that can be used for pain control.

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来源期刊

Neuroscience Letters 医学-神经科学

CiteScore

5.20

自引率

0.00%

发文量

408

审稿时长

50 days

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