黄连汤通过调节内质网应激预防和治疗辐射性肺癌肠损伤。

IF 2.5 4区 医学 Q3 CHEMISTRY, MEDICINAL
Liang-liang Shi, Ya-ping Chen, Hao-ming Guo, Xue-peng Wang, Yang-yang Li, Shuai-zhe Wang, Shang-zu Zhang, Qi-hong Zhuo, Yong-qi Liu, Ben-jun Wei, Li-ying Zhang
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引用次数: 0

摘要

放射治疗是肺癌的主要治疗方法,放射性肺损伤已被广泛研究。然而,肺和肠是密切相关的,接受肺部放疗的患者经常会出现胃肠道反应。黄连汤对肠道疾病的治疗已被证实有效,但其在肺癌放射性肠道损伤中的作用尚未得到证实。本研究探讨黄连汤对放射性肺癌肠损伤的潜在保护机制。通过体内实验观察其形态学变化。内质网应激相关蛋白的变化通过电子显微镜、免疫组织化学、免疫荧光和Western blotting进行评估。体外研究通过慢病毒载体在NCM460细胞中过表达TRPA1蛋白。荷瘤小鼠放疗后肺和结肠组织均出现严重损伤,IL-33水平升高,结肠组织中ST2L和TRPA1表达增加,内质网应激相关蛋白水平升高,细胞凋亡和炎症反应出现。黄连汤通过降低IL-33水平减轻辐射引起的肠道损伤,从而降低结肠组织内质网应激反应。在TRPA1过表达的NCM460细胞中,黄连汤降低TRPA1表达水平,显著缓解内质网应激反应。综上所述,黄连汤通过降低IL-33水平,进而抑制结肠组织中ST2L和TRPA1的表达,缓解辐射诱导的肠内质网应激。这证明了黄连汤在肺癌放疗期间对肠道的保护作用,突出了其在放射防护方面的临床应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Huanglian decoction prevents and treats radiation-induced intestinal injury in lung cancer by regulating endoplasmic reticulum stress

Radiotherapy is a primary treatment method for lung cancer, and radiation-induced lung injury has been widely studied. However, the lung and intestines are closely related, and patients receiving lung radiotherapy often experience gastrointestinal reactions. Huanglian Decoction has proven effective in treating intestinal diseases, but its role in radiation-induced intestinal injury in lung cancer has not yet been demonstrated. The study investigated the potential protective mechanisms of Huanglian Decoction against radiation-induced intestinal injury in lung cancer. In vivo experiments were conducted to examine the morphological changes. Changes in endoplasmic reticulum stress-related proteins were assessed using electron microscopy, immunohistochemistry, immunofluorescence, and Western blotting. In vitro studies involved the overexpression of TRPA1 protein in NCM460 cells via lentiviral vectors. Tumor-bearing mice exhibited severe damage to both lung and colon tissues following radiotherapy, with elevated levels of IL-33, increased expression of ST2L and TRPA1 in colon tissues, higher levels of endoplasmic reticulum stress-related proteins, and the presence of apoptosis and inflammatory responses. Huanglian Decoction reduced radiation-induced intestinal injury by lowering IL-33 levels, which in turn reduced endoplasmic reticulum stress response in colon tissues. In TRPA1-overexpressing NCM460 cells, Huanglian Decoction decreased TRPA1 expression levels and significantly alleviated endoplasmic reticulum stress response. Conclusively, the results indicate that Huanglian Decoction alleviates radiation-induced intestinal endoplasmic reticulum stress by reducing IL-33 levels, subsequently inhibiting the expression of ST2L and TRPA1 in colon tissues. This demonstrates the protective effect of Huanglian Decoction on the intestines during lung cancer radiotherapy, highlighting its promising clinical application in radiation protection.

Graphical Abstract

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来源期刊
CiteScore
6.90
自引率
3.00%
发文量
79
审稿时长
1.7 months
期刊介绍: The Journal of Natural Medicines is an international journal publishing original research in naturally occurring medicines and their related foods and cosmetics. It covers: -chemistry of natural products -biochemistry of medicinal plants -pharmacology of natural products and herbs, including Kampo formulas and traditional herbs -botanical anatomy -cultivation of medicinal plants. The journal accepts Original Papers, Notes, Rapid Communications and Natural Resource Letters. Reviews and Mini-Reviews are generally invited.
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