没食子酸包封PAMAM树状大分子作为抗氧化递送系统的控释和降低对ARPE-19细胞的细胞毒性。

IF 4 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Bioconjugate Chemistry Pub Date : 2024-12-18 Epub Date: 2024-12-06 DOI:10.1021/acs.bioconjchem.4c00475
Aorada Sripunya, Chuda Chittasupho, Supachoke Mangmool, Alexander Angerhofer, Witcha Imaram
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引用次数: 0

摘要

聚(氨基胺)(PAMAM)树状大分子在各个研究领域,特别是在药用化合物的传递方面受到了广泛的关注。它们的多功能性在于它们能够与表面的功能分子结合并封装小分子,使它们适合各种应用。没食子酸是一种有效的抗氧化化合物,近年来引起了相当大的兴趣。我们的研究目的是研究没食子酸包封的PAMAM树状分子第4代(G4(OH)-Ga)和第5代(G5(OH)-Ga)是否可以增强自由基清除能力,从而可能减缓年龄相关性黄斑变性(AMD)的进展。没食子酸包封在PAMAM树状大分子中是一种可行的替代方法,可以防止其降解和毒性。体外抗氧化活性研究采用DPPH和ABTS自由基清除实验,以及FRAP实验。DPPH和ABTS的IC50值通过非线性剂量-响应曲线确定,抑制百分比与样品浓度(μg/mL)和样品中没食子酸浓度(μM)相关。G4(OH)-Ga和G5(OH)-Ga具有显著的抗氧化活性,通过DPPH、ABTS和FRAP测定。此外,没食子酸包被的PAMAM树状大分子抑制h2o2诱导的人视网膜色素上皮ARPE-19细胞中活性氧(ROS)的产生,从而改善抗氧化特性,并可能延缓ROS引起的AMD进展。在使用MTT法评估ARPE-19细胞的细胞活力时,发现G4(OH)-Ga降低了对ARPE-19细胞的细胞毒性作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gallic Acid-Encapsulated PAMAM Dendrimers as an Antioxidant Delivery System for Controlled Release and Reduced Cytotoxicity against ARPE-19 Cells.

Poly(amidoamine) (PAMAM) dendrimers have gained significant attention in various research fields, particularly in medicinal compound delivery. Their versatility lies in their ability to conjugate with functional molecules on their surfaces and encapsulate small molecules, making them suitable for diverse applications. Gallic acid is a potent antioxidant compound that has garnered considerable interest in recent years. Our research aims to investigate if the gallic acid-encapsulated PAMAM dendrimer generations 4 (G4(OH)-Ga) and 5 (G5(OH)-Ga) could enhance radical scavenging, which could potentially slow down the progression of age-related macular degeneration (AMD). Encapsulation of gallic acid in PAMAM dendrimers is a feasible alternative to prevent its degradation and toxicity. In vitro investigation of antioxidant activity was carried out using the DPPH and ABTS radical scavenging assays, as well as the FRAP assay. The IC50 values for DPPH and ABTS assays were determined through nonlinear dose-response curves, correlating the inhibition percentage with the concentration (μg/mL) of the sample and the concentration (μM) of gallic acid within each sample. G4(OH)-Ga and G5(OH)-Ga possess significant antioxidant activities as determined by the DPPH, ABTS, and FRAP assays. Moreover, gallic acid-encapsulated PAMAM dendrimers inhibit H2O2-induced reactive oxygen species (ROS) production in the human retinal pigment epithelium ARPE-19 cells, thereby improving antioxidant characteristics and potentially retarding AMD progression caused by ROS. In an evaluation of cell viability of ARPE-19 cells using the MTT assay, G4(OH)-Ga was found to reduce cytotoxic effects on ARPE-19 cells.

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来源期刊
Bioconjugate Chemistry
Bioconjugate Chemistry 生物-化学综合
CiteScore
9.00
自引率
2.10%
发文量
236
审稿时长
1.4 months
期刊介绍: Bioconjugate Chemistry invites original contributions on all research at the interface between man-made and biological materials. The mission of the journal is to communicate to advances in fields including therapeutic delivery, imaging, bionanotechnology, and synthetic biology. Bioconjugate Chemistry is intended to provide a forum for presentation of research relevant to all aspects of bioconjugates, including the preparation, properties and applications of biomolecular conjugates.
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