一株解淀粉芽孢杆菌大乳清蛋白A的分离及其对帕氏蚜的杀虫活性

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-12-06 DOI:10.1002/ps.8589

Kangbo Zhao, Ruyi Ma, Min Cheng, Ting Guo, Wenjun Wu, Yuxin Song, Hong Xu, Aoping Tan, Baofu Qin, Shaopeng Wei

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引用次数: 0

摘要

水稻粉霉（Rhopalosiphum padi）是边界地区影响谷类作物的主要病害。然而，化学农药的持续使用增加了其田间种群的耐药性。因此，我们旨在验证解淀粉芽孢杆菌YJNbs21.10对蚜虫的杀虫性能，并首次测定其代谢物大乳清素A对蚜虫的生物活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Isolation of Macrolactin A from a new Bacillus amyloliquefaciens and its aphicidal activity against Rhopalosiphum padi

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Isolation of Macrolactin A from a new Bacillus amyloliquefaciens and its aphicidal activity against Rhopalosiphum padi

BACKGROUND

Rhopalosiphum padi is a major infection affecting cereal crops in the boundary area. However, continuous use of chemical pesticides has increased cases of drug resistance in its field population. Therefore, we aimed to verify the insecticidal properties of Bacillus amyloliquefaciens YJNbs21.10 against aphids, isolated to determine the bioactivity of its metabolite Macrolactin A against aphids for the first time.

RESULTS

The results of activity tracking showed that the fermentation broth of YJNbs21.10 had the best inhibitory efficacy against R. padi, and the corrected efficiency reached 95.57% after 24 h. With the continuous separation and test, the efficiency of the active components decreased: Macrolactin A, as the most active substance, had a control activity against aphids under 500 mg L⁻¹ of 74.64% at 72 h, (which was significantly lower than that of fermentation broth, indicating a synergistic effect between the active substances of each part of the strain. In addition, the result of the stereomicroscope showed that Macrolactin A damaged the body wall of aphids. The toxicity of Macrolactin A to R. padi was confirmed through the gradient test.

CONCLUSION

In this study, Bacillus amyloliquefaciens YJNbs21.10 exhibited comparable inhibitory ability to chemical pesticides suggesting its potential to provide effective biological control on aphids. The biological activity of Macrolactin A against aphids was also verified for the first time, in this experiment, the EC₅₀ of this substance against aphids was 169.02 mg L⁻¹ (24 h), which provided strong evidence that YJNbs21.10 may act as an effective agent for the prevention of aphid. © 2024 Society of Chemical Industry.

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.