中国南方传统牡蛎晒干过程中微生物组和代谢组的动态变化

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Journal of microbiology and biotechnology Pub Date : 2024-12-28 Epub Date: 2024-07-17 DOI:10.4014/jmb.2312.12033
Jiannan Liu, Mingjia Yu, Xiaobo Wang, Ming Qi, Minfu Wu
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引用次数: 0

摘要

在华南沿海地区,太阳晒干是制备干牡蛎的一种传统方法。然而,它对营养属性的影响以及由此产生的干牡蛎中风味贡献化合物的成因仍未得到充分研究。本研究旨在探讨生产过程对干牡蛎内微生物群和代谢物的影响。利用16s rRNA扩增子测序,鉴定出409个操作分类单位(OTUs),其中变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、微内菌门(Tenericutes)和放线菌门(Actinobacteria)是牡蛎样品中的主要致病菌。通过LC-MS对干燥牡蛎样品代谢组的分析发现,在整个干燥过程中,与类固醇激素生物合成、花生四烯酸代谢、不饱和脂肪酸生物合成和亚油酸代谢相关的化合物明显增加。随后对代谢物与细菌群落之间关系的探索强调了支原体、精神杆菌和弧菌的普遍共存,表明它们与大量代谢物呈负相关,包括有机酸及其衍生物、核苷酸及其代谢物、游离脂肪酸和氨基酸。相反,希瓦氏菌和Arcobacter与这些代谢物类别呈正相关。这项详尽的调查提供了宝贵的见解,在不同的干燥时期的干牡蛎的微生物群和代谢物的动态变化。预期这些发现将大大促进生产技术的进步和为加工干牡蛎制定加强的安全措施。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dynamic Changes in Microbiome and Metabolome during Sun-Drying of Oysters (Crassostrea gigas), a Traditional Procedure in South China.

Sun-drying constitutes a traditional method employed in the preparation of dried oysters within the coastal regions of South China. However, its ramifications on nutritional attributes and the genesis of flavor-contributory compounds in the resultant dried oysters remain significantly unexplored. This research endeavors to scrutinize the repercussions of the production process on the microbiota and metabolites within dried oysters. Utilizing 16s rRNA amplicon sequencing, the identification of 409 operational taxonomic units (OTUs) ensued, wherein Proteobacteria, Bacteroidetes, Firmicutes, Tenericutes, and Actinobacteria surfaced as the primary pathogenic bacteria present in oyster samples. Analysis of the dried oyster sample metabolomes via LC-MS unveiled a discernible augmentation in compounds associated with steroid hormone biosynthesis, arachidonic acid metabolism, biosynthesis of unsaturated fatty acids, and linoleic acid metabolism throughout the entirety of the drying process. Subsequent exploration into the association between metabolites and bacterial communities highlighted the prevailing coexistence of Mycoplasma, Psychrilyobacter, and Vibrio demonstrating negative correlations with a substantial number of metabolites across categories including organic acid and its derivatives, nucleotide and its metabolites, free fatty acids, and amino acids. Conversely, Shewanella and Arcobacter exhibited positive correlations with these metabolite categories. This exhaustive investigation offers invaluable insights into the dynamic alterations within the microbiota and metabolites of dried oysters across diverse drying periods. These findings are anticipated to significantly contribute to the advancement of production techniques and the formulation of enhanced safety measures for the processing of dried oysters.

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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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