条件小鼠实验中对cre位点对照的需要：Mrp8-cre转基因使小鼠易患抗体诱导的关节炎。

IF 5 3区医学 Q1 GENETICS & HEREDITY

Genes and immunity Pub Date : 2024-12-04 DOI:10.1038/s41435-024-00313-3

Zhongwei Xu, Laura Romero-Castillo, Àlex Moreno-Giró, Rajan Kumar Pandey, Rikard Holmdahl

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引用次数: 0

摘要

Cre/loxP系统被广泛用于确定特定细胞类型或发育阶段的基因功能，通常不会对宿主基因组造成重大干扰。然而，我们发现随机插入Mrp8-cre转基因可显著促进宿主的先天免疫反应。这种作用的特点是对软骨抗体诱导的关节炎的易感性增加，可能是由于插入部位附近基因的干扰。这些发现强调了随机转基因整合引起的潜在生物学干扰，以及在使用cre条件菌株时需要严格的控制策略以避免有偏见的解释。

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The need for Cre-loci controls in conditional mouse experiments: Mrp8-cre transgene predisposes mice to antibody-induced arthritis.

The Cre/loxP system is extensively utilized to pinpoint gene functions in specific cell types or developmental stages, typically without major disturbance to the host's genome. However, we found that the random insertion of the Mrp8-cre transgene significantly promotes the host's innate immune response. This effect is characterized by elevated susceptibility to cartilage antibody-induced arthritis, likely due to interference with genes near the insertion site. These findings underscore the potential biological disturbances caused by random transgene integration, and the necessity for stringent control strategies to avoid biased interpretations when using Cre-conditional strains.

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来源期刊

Genes and immunity 医学-免疫学

CiteScore

8.90

自引率

4.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Genes & Immunity emphasizes studies investigating how genetic, genomic and functional variations affect immune cells and the immune system, and associated processes in the regulation of health and disease. It further highlights articles on the transcriptional and posttranslational control of gene products involved in signaling pathways regulating immune cells, and protective and destructive immune responses.