通过多生物标志物方法评估贻贝（Mytilus galloprovincialis）鳃中实际地塞米松浓度引起的细胞通路干扰。

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-01-01 DOI:10.1016/j.etap.2024.104598

Giuseppe De Marco , Antonio Cristaldi , Maria Concetta Eliso , Gea Oliveri Conti , Mariachiara Galati , Barbara Billè , Mery Terranova , Vincenzo Parrino , Tiziana Cappello , Margherita Ferrante , Maria Maisano

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引用次数: 0

摘要

糖皮质激素越来越多地用于治疗各种疾病，这引起了人们的关注，因为这些药物，包括抗炎药物地塞米松（DEX），经常在环境中发现。通过暴露于环境浓度(C1: 4ng/L；C2: 40 ng / L;C3: 400 ng / L;C4: 2000 ng/L)，并在3 (T3), 6 （T6）和12 （T12）天取样。多生物标志物的方法应用于鳃，涉及气体交换，饲料过滤和渗透调节。DEX的剂量和时间依赖性摄取记录，除了血细胞浸润外，中性和酸性粘多糖增加，以及脂质过氧化和抗氧化系统改变的一般促氧化作用。代谢组学显示，由于游离氨基酸（丙氨酸、甘氨酸）和能量相关代谢物（琥珀酸盐、ATP/ADP）的波动，蛋白质周转和能量需求增加。有必要通过恢复策略和有效的生态药物警戒计划从环境中减少DEX的剂量。

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Cellular pathway disturbances elicited by realistic dexamethasone concentrations in gills of mussel Mytilus galloprovincialis as assessed by a multi-biomarker approach

The growing usage of glucocorticoids for a variety of diseases raises concerns since these drugs, including the anti-inflammatory dexamethasone (DEX), are frequently found in the environment. The impact of DEX was evaluated on mussels Mytilus galloprovincialis (Lamarck, 1819) by exposure to environmental concentrations (C1: 4 ng/L; C2: 40 ng/L; C3: 400 ng/L; C4: 2000 ng/L), and sampling at 3 (T3), 6 (T6), and 12 (T12) days. A multi-biomarker approach was applied on gills, involved in gas exchange, feed filtering, and osmoregulation. A dose- and time-dependent uptake of DEX was recorded, besides haemocyte infiltration, increased neutral and acid mucopolysaccharides, and a general pro-oxidant effect witnessed by lipid peroxidation and altered antioxidant system. Metabolomics revealed rise in protein turnover and energy demand by fluctuations in free amino acids (alanine, glycine) and energy-related metabolites (succinate, ATP/ADP). It is necessary to reduce DEX dosage from the environment by recovery strategies and effective eco-pharmacovigilance programs.

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.