通过qBOLD MRI评估胶质母细胞瘤缺氧及其动态演变:与二甲双胍治疗的比较研究。

IF 3.7 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Dongdong Wang, Jie Chen, Yinwei Ying, Xinxin Zhao, Nan Mei, Xuanxuan Li, Yuqi Zhu, Jin Cui, Pu-Yeh Wu, Yiping Lu, Bo Yin
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引用次数: 0

摘要

背景:探讨定量血氧水平依赖(qBOLD)磁共振成像(MRI)识别胶质母细胞瘤内缺氧的准确性,探讨使用和不使用二甲双胍时胶质母细胞瘤氧合状态的动态变化。方法:3只健康大鼠和7只产c6大鼠行7-T qBOLD MRI检查。根据qBOLD数据计算脑氧提取分数(OEF)和脑氧代谢率(cdro2)。用缺氧诱导因子-1 α (HIF-1 α)和吡莫硝唑对肿瘤组织进行染色。分析缺氧标志物与相应的qbold参数之间的相关性。6只携带c6的大鼠分为二甲双胍治疗组和对照组,在肿瘤植入后第12、15、18天进行qBOLD影像学变化的纵向研究。结果:健康大鼠T2、T2*、脑血容量(CBV)、脑血流量(CBF)灰质值高于白质,OEF值低于白质。胶质母细胞瘤组织表现为T2、T2*、CBV和CBF升高,但OEF和cmor2相对于正常的白质降低。HIF-1 α染色评分与吡莫硝唑染色评分无显著相关性。T2*、T2值与肿瘤区域吡莫硝唑评分呈负相关。随着肿瘤的进展,OEF值随着组织内变化而增加,而cmor2则下降。二甲双胍延缓了T2和T2*值的降低,第18天OEF和cmoro2值与对照组相比有显著差异。结论:T2*、T2值与胶质瘤缺氧状态有显著相关性。二甲双胍可能潜在地减缓胶质母细胞瘤缺氧的进展,这可以通过qBOLD参数跟踪。相关声明:本研究证明了qBOLD参数在评估胶质瘤动态氧代谢和二甲双胍作为抗缺氧药物的疗效方面的潜力,为改善胶质母细胞瘤的治疗策略提供了见解。重点:本研究探讨了qBOLD成像识别胶质母细胞瘤内缺氧状态的准确性。qBOLD可有效评估胶质母细胞瘤缺氧及其动态演变。qBOLD参数有助于确定适合二甲双胍治疗的患者人群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of hypoxia and its dynamic evolution in glioblastoma via qBOLD MRI: a comparative study with metformin treatment.

Background: To investigate the accuracy of quantitative blood oxygen level-dependent (qBOLD) magnetic resonance imaging (MRI) in identifying hypoxia within glioblastoma and explore dynamic changes in oxygenation status of glioblastoma with and without metformin administration.

Methods: Three healthy and seven C6-bearing rats underwent 7-T qBOLD MRI. Oxygen extraction fraction (OEF) and cerebral metabolism rate of O2 (CMRO2) were calculated from qBOLD data. Tumor tissues were stained using hypoxia-inducible factor-1 α (HIF-1 α ) and pimonidazole. The correlation between the hypoxia markers and corresponding qBOLD-based parameters was analyzed. Six C6-bearing rats were divided into metformin-treated and control groups for a longitudinal study of qBOLD imaging changes, with scans conducted on the 12th, 15th, and 18th day post-tumor implantation.

Results: In healthy rats, gray matter showed higher values than white matter in T2, T2*, cerebral blood volume (CBV), and cerebral blood flow (CBF), whereas OEF was lower. Glioblastoma tissues exhibited elevated T2, T2*, CBV, and CBF but decreased OEF and CMRO2 relative to normal-appearing white matter. No significant correlation was found between staining scores from HIF-1 α and pimonidazole. T2* and T2 values were negatively correlated with pimonidazole scores in tumor regions. As the tumor progressed, OEF values increased with intra-tissue variations, whereas CMRO2 decreased. Metformin delayed the reduction of T2 and T2* values, with significant differences in OEF and CMRO2 values compared to controls on day 18.

Conclusion: T2* and T2 values were significantly associated with the hypoxia status in glioma. Metformin could potentially mitigate the progression of hypoxia in glioblastoma, which can be tracked by qBOLD parameters.

Relevance statement: This study demonstrates the potential of qBOLD parameters in assessing glioma dynamic oxygen metabolism and the efficacy of metformin as an anti-hypoxic agent, providing insights into improving glioblastoma treatment strategies.

Key points: The study investigated qBOLD imaging's accuracy in identifying hypoxia status within glioblastoma. qBOLD effectively assesses hypoxia and its dynamic evolution in glioblastoma. qBOLD parameters assist in identifying a suitable patient demographic for metformin treatment.

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来源期刊
European Radiology Experimental
European Radiology Experimental Medicine-Radiology, Nuclear Medicine and Imaging
CiteScore
6.70
自引率
2.60%
发文量
56
审稿时长
18 weeks
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