19F-PCS对活哺乳动物细胞中蛋白质的测量

IF 4.2 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2024-12-13 DOI:10.1039/d4cc05667a

Shu-Li Guo , Bin-Bin Pan , Xia-Yan Li , Yu-Hao Xiao , Xun-Cheng Su

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引用次数: 0

摘要

在结构生物学和化学生物学中，赝接触位移（PCS）包含了丰富的蛋白质结构信息。在这里，19F-PCS是通过用顺磁标签和19f标签对靶蛋白进行双重标记在活的哺乳动物细胞中实现的。约0.1 ppm 19F-PCS可在20 Å以上从活细胞的顺磁中心测定。这是第一次在活的哺乳动物细胞中观察到19F-PCS。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

19F-PCS measurements on proteins in live mammalian cells†

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19F-PCS measurements on proteins in live mammalian cells†

Pseudocontact shift (PCS) contains rich structural information of proteins in structural and chemical biology. ¹⁹F-PCS is determined in live mammalian cells via dual labelling of the target protein with a paramagnetic tag and a ¹⁹F-tag, which is achieved by varied reactivity of solvent exposed cysteines in selection of different types of tags. About 0.1 ppm ¹⁹F-PCS can be observed over 20 Å from the paramagnetic center in the live cells.

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.