妊娠期空气污染暴露对单细胞水平细胞因子和组蛋白修饰谱的影响。

IF 12.5 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2024-11-29 DOI:10.1126/sciadv.adp5227

Youn Soo Jung, Juan Aguilera, Abhinav Kaushik, Ji Won Ha, Stuart Cansdale, Emily Yang, Rizwan Ahmed, Fred Lurmann, Liza Lutzker, S. Katherine Hammond, John Balmes, Elizabeth Noth, Trevor D. Burt, Nima Aghaeepour, Anne R. Waldrop, Purvesh Khatri, Paul J. Utz, Yael Rosenburg-Hasson, Rosemarie DeKruyff, Holden T. Maecker, Mary M. Johnson, Kari C. Nadeau

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引用次数: 0

摘要

细颗粒物（PM2.5）暴露可通过表观遗传修饰诱导免疫系统病变，影响妊娠结局。本研究利用单细胞飞行时间（EpiTOF）细胞术的高维表观遗传景观分析研究了PM2.5暴露与免疫反应之间的关系，以及表观遗传变化。我们发现PM2.5暴露与孕妇和非孕妇免疫细胞（HPTMs: H3K9ac、H3K23ac、H3K27ac、H2BK120ub、H4K20me1/3和H3K9me1/2）中某些细胞因子[白介素- 1ra （IL-1RA）、IL-8/CXCL8、IL-18和IL-27)]和组蛋白翻译后修饰（HPTMs）水平之间存在统计学意义上的关联。PM2.5暴露量高的新生儿脐带血IL-27含量低于暴露量低的新生儿。此外，PM2.5暴露会影响孕妇及其新生儿之间细胞因子的共修饰谱，以及孕妇和非孕妇之间每种免疫细胞类型的HPTMs。这些特异性组蛋白和细胞因子的改变可能提示PM2.5暴露在炎症、炎症小体途径和妊娠并发症中的毒理学机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Impact of air pollution exposure on cytokines and histone modification profiles at single-cell levels during pregnancy

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Impact of air pollution exposure on cytokines and histone modification profiles at single-cell levels during pregnancy

Fine particulate matter (PM_2.5) exposure can induce immune system pathology via epigenetic modification, affecting pregnancy outcomes. Our study investigated the association between PM_2.5 exposure and immune response, as well as epigenetic changes using high-dimensional epigenetic landscape profiling using cytometry by time-of-flight (EpiTOF) at the single cell. We found statistically significant associations between PM_2.5 exposure and levels of certain cytokines [interleukin-1RA (IL-1RA), IL-8/CXCL8, IL-18, and IL-27)] and histone posttranslational modifications (HPTMs) in immune cells (HPTMs: H3K9ac, H3K23ac, H3K27ac, H2BK120ub, H4K20me1/3, and H3K9me1/2) among pregnant and nonpregnant women. The cord blood of neonates with high maternal PM_2.5 exposure showed lower IL-27 than those with low exposure. Furthermore, PM_2.5 exposure affects the co-modification profiles of cytokines between pregnant women and their neonates, along with HPTMs in each immune cell type between pregnant and nonpregnant women. These modifications in specific histones and cytokines could indicate the toxicological mechanism of PM_2.5 exposure in inflammation, inflammasome pathway, and pregnancy complications.

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.