定量评估HSPCs和骨髓生态位生物物理特性随年龄变化的新技术。

IF 2.5 4区 医学 Q2 HEMATOLOGY
Anthony D. Ho , Motomu Tanaka
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引用次数: 0

摘要

目前关于造血干细胞和祖细胞(HSPC)生物学和衰老的知识主要是基于小鼠模型的研究。虽然小鼠模型是无价的,但它们在定义HSPCs的物理特性及其生态位随年龄变化的情况下并非没有限制。骨髓(BM)生态位是一个复杂的、与多种细胞类型相互作用的环境。BM生态位的结构和组织,尤其是细胞外基质(ECM),随着年龄的增长而变化。利用定量分析技术和体外生态位模型的最新进展,我们开发了新的工具来定量评估特定生化和物理线索对HSPCs归巢、粘附和迁移的影响。体外生态位模型的最新进展也为HSPCs与其生态位之间的相互作用,特别是基质刚度的作用提供了新的见解。需要进一步研究将物理生物标志物整合到年龄依赖性hspc -生态位相互作用的综合数学模型中。关键是将小鼠模型与体外生态位模型的直接分析相结合,以更全面地了解生态位功能和调节的年龄依赖性改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Novel techniques to quantitatively assess age-dependent alterations in biophysical properties of HSPCs and bone marrow niche
The present knowledge on hematopoietic stem and progenitor cell (HSPC) biology and aging is based largely on studies in mouse models. Although mouse models are invaluable, they are not without limitations for defining how physical properties of HSPCs and their niche change with age. The bone marrow (BM) niche is a complex, interactive environment with multiple cell types. The structure and organization of the BM niche, especially the extracellular matrix (ECM), change with age. Provided with recent advances in quantitative analytical techniques and in vitro niche models, we have developed novel tools to quantitatively assess the impact of specific biochemical and physical cues on homing, adhesion, and migration of HSPCs. Recent developments in in vitro niche models have also provided new insights into the interactions between HSPCs and their niche, particularly the role of matrix stiffness. Further research is needed to integrate physical biomarkers into comprehensive mathematical models of age-dependent HSPC-niche interactions. The key is to use mouse models in conjunction with direct analyses in in vitro niche models to achieve a more comprehensive understanding of age-dependent alterations in niche function and regulation.
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来源期刊
Experimental hematology
Experimental hematology 医学-血液学
CiteScore
5.30
自引率
0.00%
发文量
84
审稿时长
58 days
期刊介绍: Experimental Hematology publishes new findings, methodologies, reviews and perspectives in all areas of hematology and immune cell formation on a monthly basis that may include Special Issues on particular topics of current interest. The overall goal is to report new insights into how normal blood cells are produced, how their production is normally regulated, mechanisms that contribute to hematological diseases and new approaches to their treatment. Specific topics may include relevant developmental and aging processes, stem cell biology, analyses of intrinsic and extrinsic regulatory mechanisms, in vitro behavior of primary cells, clonal tracking, molecular and omics analyses, metabolism, epigenetics, bioengineering approaches, studies in model organisms, novel clinical observations, transplantation biology and new therapeutic avenues.
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