Vatsal Bhargava, Darshana Lahon, Sonal Gupta, Jasvinder Kaur, Pushp Lata
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引用次数: 0
摘要
本研究是为了阐明两个密切相关的螺原体种的分类归属。在进行这项分析时，有26种类型菌株的基因组信息可用。我们的分析表明，有两个物种，即猪螺旋体（Koerber et al. 2005）和微小螺旋体（Tully et al. 1982）是同源的。两种的16S rRNA基因序列相似性达99.78%。此外，全基因组序列比较表明，非洲血吸虫GNAT3597T (CP011855.1) GCA 001029245.1和S. mirum ATCC 29335T (CP002082.1) GCA 000517365.1具有99.99%的平均核苷酸同源性、99.99%的平均氨基酸同源性和100%的数字DNA-DNA杂交值。这些值超过了细菌种类划分的阈值，表明它们属于同一种。此外，基于核心蛋白质组的系统基因组分析证实，S. atrichopogonis GNAT3597T (CP011855.1) GCA 001029245.1与S. mirum ATCC 29335T (CP002082.1) GCA 000517365.1为单系进化分支。基于这一证据，我们建议将S. atrichopogonis Koerber et al. 2005重新分类为S. mirum Tully et al. 1982的后异型同义种。
Genome-based reclassification of Spiroplasma atrichopogonis Koerber et al. 2005 as a later heterotypic synonym of Spiroplasma mirum Tully et al. 1982.
The present study was carried out to clarify the taxonomic assignment of two closely related Spiroplasma species. Genomic information for 26 type strains was available at the time of conducting this analysis. Our analysis showed that two species, viz. Spiroplasma atrichopogonis (Koerber et al. 2005) and Spiroplasma mirum (Tully et al. 1982), are conspecific. The 16S rRNA gene sequences of the two species possess 99.78% sequence similarity. Furthermore, whole-genome sequence comparisons showed that S. atrichopogonis GNAT3597T (CP011855.1) GCA 001029245.1 and S. mirum ATCC 29335T (CP002082.1) GCA 000517365.1 shared 99.99% average nucleotide identity, 99.99% average amino acid identity and 100% digital DNA-DNA hybridization values. These values exceed the threshold values for bacterial species delineation, indicating that they belong to the same species. Furthermore, the phylogenomic analysis based on the core proteome of the strains under study confirmed that S. atrichopogonis GNAT3597T (CP011855.1) GCA 001029245.1 and S. mirum ATCC 29335T (CP002082.1) GCA 000517365.1 formed a monophyletic clade. Based on this evidence, we propose the reclassification of S. atrichopogonis Koerber et al. 2005 as a later heterotypic synonym of S. mirum Tully et al. 1982.
期刊介绍：
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.
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