以维生素B6辅助因子为交联剂的聚乙烯亚胺基荧光聚合物纳米棒的快速合成用于比例检测阿霉素。

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-11-29 DOI:10.1007/s00604-024-06856-6

Anuj K Saini, Ritambhara Jangir, Bigyan Ranjan Jali, Suban K Sahoo

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引用次数: 0

摘要

以维生素B6辅助因子5′-磷酸吡哆醛（PLP）为原料，通过交联和自组装制备了高量子产率（87.21%）的聚乙烯亚胺（PEI）基荧光聚合物纳米棒。采用pei - plp荧光聚合物纳米棒对阿霉素（DOX）进行了比例检测。在PEIPLP纳米棒中加入DOX后，由于荧光共振能量转移（FRET）过程，在510 nm处荧光强度降低，在590 nm处荧光强度增强。PEIPLP纳米棒的选择性和特异性比例荧光响应可用于检测低至10 nM的DOX。通过检测人血清中的DOX来检验PEIPLP纳米棒的实用性。

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A facile synthesis of polyethyleneimine based fluorescent polymeric nanorods using vitamin B6 cofactor as cross-linker for ratiometric detection of doxorubicin

Polyethyleneimine (PEI) based fluorescent polymeric nanorods with high quantum yield (87.21%) were synthesized by crosslinking and self-assembly of PEI by vitamin B₆ cofactor pyridoxal 5’-phosphate (PLP). The fluorescent PEIPLP polymeric nanorods were employed for the ratiometric detection of doxorubicin (DOX). With the addition of DOX to the PEIPLP nanorods, the fluorescent intensity was decreased at 510 nm and concomitantly enhanced at 590 nm due to the fluorescence resonance energy transfer (FRET) process. The selective and specific ratiometric fluorescence response from the PEIPLP nanorods can be employed to detect DOX down to 10 nM. The practical utility of the PEIPLP nanorods was examined by detecting DOX in human serum.

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.