IF 2.2 3区 医学 Q1 MEDICINE, LEGAL
Milica Marković , Miroslav Kuzmanović , Igor Pašti , Danica Bajuk-Bogdanović , Dragan Ranković , Dušan Dimić
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引用次数: 0

摘要

通过 ATR-傅立叶变换红外光谱、拉曼光谱、LIBS 光谱和 SEM-EDX 技术监测了在盐酸、氢氟酸和醋酸中经过化学处理的猪肩骨的脱矿情况。SEM-EDX 分析表明,与保存在水中的样本相比,经酸处理后的钙和磷含量降低,骨骼的整体形态受到侵蚀。在酸溶液中长达 14 天的浸泡期间,骨结构发生了变化,这表明所获得的光谱发生了显著的化学变化。在酰胺 I(1700-1600 cm-1)、磷酸盐(900-1200 cm-1)和碳酸盐(500-650 cm-1)区域应用的傅立叶解卷积表明,根据环境和酸浓度的不同,骨样本中存在不同的成分,从而提供了有关成分的信息。利用 ATR-FTIR 和拉曼数据计算并比较了矿物与基质比率、结晶度指数和碳酸盐与磷酸盐比率等参数。这些参数还与 LIBS 光谱中获得的钙离子-原子线强度和磷-碳线强度相关联。通过 SEM-EDX 分析获得的钙和磷原子含量与 LIBS 数据一致。结果表明,酸浓度的增加主要影响了磷酸盐带的强度和结构,因为磷酸盐含量更容易脱矿。与氢氟酸和醋酸相比,盐酸被证明是一种更强的脱矿剂。这项研究的结果可进一步应用于犯罪现场骨骼残骸的调查,尤其是在试图通过浸泡在酸溶液中去除骨骼残骸时。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comprehensive spectroscopic and morphological analysis of the effects exerted by different acids on Pig bone: Forensic aspect
Demineralization of the chemically treated pig shoulder bone in hydrochloric, hydrofluoric, and acetic acid was monitored by ATR-FTIR, Raman, and LIBS spectroscopies and SEM-EDX technique. SEM-EDX analysis showed reduced calcium and phosphorus content after the treatment with acids and erosion of the overall morphology of the bone compared to the sample kept in water. Alterations in bone structure during the 14-day-long immersion in acid solutions indicated significant chemical changes in the obtained spectra. Fourier deconvolution applied in the amide I (1700–1600 cm−1), phosphate (900–1200 cm−1), and carbonate (500–650 cm−1) region indicated the presence of different components in the bone sample, depending on the environment and acid concentration, providing information about the composition. Parameters such as mineral-to-matrix ratio, crystallinity index, and carbonate-to-phosphate ratio were calculated and compared using ATR-FTIR and Raman data. These parameters were also correlated with calcium ionic-to-atomic and phosphorous-to-carbon line intensities obtained from LIBS spectra. Calcium and phosphorus atomic contents obtained by SEM-EDX analysis were in agreement with LIBS data. The results suggested that an increase in acid concentration has primarily affected the phosphate band’s intensity and structure, as the phosphate content was more susceptible to demineralization. Hydrochloric acid was proven to be a more powerful demineralization agent than hydrofluoric and acetic acids. The results of this study could be further applied to the investigation of the bone remains at the crime scene, especially when their removal is attempted by immersion in acid solutions.
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来源期刊
Forensic science international
Forensic science international 医学-医学:法
CiteScore
5.00
自引率
9.10%
发文量
285
审稿时长
49 days
期刊介绍: Forensic Science International is the flagship journal in the prestigious Forensic Science International family, publishing the most innovative, cutting-edge, and influential contributions across the forensic sciences. Fields include: forensic pathology and histochemistry, chemistry, biochemistry and toxicology, biology, serology, odontology, psychiatry, anthropology, digital forensics, the physical sciences, firearms, and document examination, as well as investigations of value to public health in its broadest sense, and the important marginal area where science and medicine interact with the law. The journal publishes: Case Reports Commentaries Letters to the Editor Original Research Papers (Regular Papers) Rapid Communications Review Articles Technical Notes.
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