当归补血汤通过调节MEK1/2/ erk1 /NF-κB通路减轻大鼠原发性痛经

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2024-11-29 DOI:10.1016/j.fitote.2024.106315

Shi-Jia Gao , Xiao-Li Li , Rui Gao , Wen-Hong Tan , Wen Li , Lu Liu

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引用次数: 0

摘要

本研究旨在探讨当归补血汤（DBD）治疗原发性痛经（PD）的疗效，揭示其作用机制，从而验证其传统应用价值。采用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF/MS）技术，鉴定了38个化合物。通过冷刺激、苯甲酸雌二醇和催产素注射建立PD模型大鼠。DBD治疗改善了行为参数，减少了扭动反应，减轻了子宫和卵巢的组织病理学变化。采用酶联免疫吸附法（ELISA）检测各组生化指标（PGE2、SOD、MDA等），Western blot检测各组大鼠子宫内蛋白水平。结果表明，DBD可调节这些蛋白，表明其具有保护作用。本研究为DBD在PD治疗过程中通过影响MEK1/2/ erk1 /NF-κB信号通路，从而降低PD大鼠氧化应激和促炎因子，起到补“气”或补血的作用提供了科学依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Danggui Buxue decoction alleviates primary dysmenorrhea in rats by regulating the MEK1/2/ERK1/2/NF-κB pathway

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Danggui Buxue decoction alleviates primary dysmenorrhea in rats by regulating the MEK1/2/ERK1/2/NF-κB pathway

The study aimed to investigate the efficacy of Danggui Buxue Decoction (DBD) in treating primary dysmenorrhea (PD) and uncover its mechanisms, thereby validating its traditional use. Using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS), 38 compounds in DBD were identified. PD model rats were established via cold stimulation, estradiol benzoate, and oxytocin injections. DBD treatment improved behavioral parameters, reduced writhing response, and alleviated histopathological changes in uterus and ovary. Biochemical markers (PGE2, SOD and MDA, among others) were assessed using ELISA, and Western blot was employed to analyze protein levels in rat uteri. Results showed that DBD modulated these proteins, indicating its protective effects. The study provides scientific evidence for DBD's role in nourishing “qi” or enriching “blood” during PD treatment by affecting the MEK1/2/ERK1/2/NF-κB signaling pathway, thus reducing oxidative stress and proinflammatory factors in PD rats.

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.