实验室确诊登革热患者尿液和口腔液中存在登革热病毒RNA：对废水监测的影响

IF 3 4区医学 Q2 INFECTIOUS DISEASES

Brazilian Journal of Infectious Diseases Pub Date : 2024-11-29 DOI:10.1016/j.bjid.2024.104484

Christine Stauber , Leile Camila Jacob-Nascimento , Caroline Grosch , Moisés da Silva Sousa , Moyra M. Portilho , Rosângela O. Anjos , Margo A. Brinton , Uriel Kitron , Mitermayer G. Reis , Guilherme S. Ribeiro

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引用次数: 0

摘要

2024年美洲的登革热病例达到历史新高，特别是在巴西。然而，监测仍然不够理想，需要新的方法来监测登革热病毒（DENV）的传播。为了评估基于废水的流行病学是否会成为一种有用的工具，我们调查了登革热流行地区登革热患者尿液和口腔液中DENV RNA的存在，以了解这些液体中的脱落是如何发生的，并为废水监测提供见解。方法对巴西萨尔瓦多地区经血清RT-qPCR、NS1 ELISA或IgM血清转化确诊的登革热患者尿液和口腔液中DENV RNA的检测频率进行检测。结果在88例确诊病例中，尿（7/88）和口服液（1/88）DENV RNA阳性率为9.1%。在53例血清rt - qpcr阳性患者中，6例（11.3%）在急性期或恢复期尿液中检测到DENV RNA。尿RT-qPCR阳性的患者在急性期血清中DENV IgG（继发感染的代理）的频率较低（57%对74%），血清中位RT-qPCR周期阈值低于尿阴性的患者（21.8对23.9）。结论尿中DENV RNA的低含量表明，需要进一步研究以废水为基础的流行病学监测DENV传播是否可行。

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查看原文本刊更多论文

Presence of dengue virus RNA in urine and oral fluid of laboratory-confirmed dengue patients: Implications for wastewater surveillance

Introduction

Dengue cases in the Americas in 2024 have reached record highs, especially in Brazil. However, surveillance remains suboptimal and new methods are needed to monitor Dengue Virus (DENV) spread. To assess whether wastewater-based epidemiology would be a useful tool, we investigated the presence of DENV RNA in dengue patients’ urine and oral fluid from an endemic area to inform how shedding in these fluids occurs and provide insight for wastewater surveillance.

Methods

We examined how often DENV RNA is detected in urine and oral fluid from dengue patients confirmed by serum RT-qPCR, NS1 ELISA or IgM seroconversion in Salvador, Brazil.

Results

Of 88 confirmed cases, 9.1 % were positive for DENV RNA in urine (7/88) or oral fluid (1/88). Of 53 serum RT-qPCR-positive patients, 6 (11.3 %) showed detectable DENV RNA in acute- or convalescent-phase urine. Patients with RT-qPCR-positive urine had a lower frequency of DENV IgG in acute-phase serum (a proxy for secondary infection) (57 % vs. 74 %) and a lower median serum RT-qPCR cycle threshold than those with negative urine (21.8 vs. 23.9).

Conclusion

The low presence of DENV RNA in urine suggests that additional research is needed to evaluate whether using wastewater-based epidemiology to monitor DENV transmission is possible.

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来源期刊

Brazilian Journal of Infectious Diseases 医学-传染病学

CiteScore

5.50

自引率

0.00%

发文量

925

审稿时长

41 days

期刊介绍： The Brazilian Journal of Infectious Diseases is the official publication of the Brazilian Society of Infectious Diseases (SBI). It aims to publish relevant articles in the broadest sense on all aspects of microbiology, infectious diseases and immune response to infectious agents. The BJID is a bimonthly publication and one of the most influential journals in its field in Brazil and Latin America with a high impact factor, since its inception it has garnered a growing share of the publishing market.