Minh Tri Luu, Jonathan F. Berengut, Jiahe Li, Jing-Bing Chen, Jasleen Kaur Daljit Singh, Kanako Coffi Dit Glieze, Matthew Turner, Karuna Skipper, Sreelakshmi Meppat, Hannah Fowler, William Close, Jonathan P. K. Doye, Ali Abbas, Shelley F. J. Wickham
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引用次数: 0
摘要
在细胞中,蛋白质可快速自组装成精密的纳米机器。受生物启发的自组装方法(如 DNA 折纸)已被用于实现复杂的三维(3D)纳米结构和器件。然而,目前的合成系统受限于分层组装的低产量,以及在不同结构之间快速高效地重新配置所面临的挑战。在这里,我们开发了一种具有可编程三维连接的 DNA 折纸 "体块 "模块化系统。我们展示了由多达 12 个独特体块组成的多功能池,这些体块可以组装成多种形状,原型结构多达 50 种。局部连接在柔性和刚性状态之间的可编程切换实现了三维全局结构的快速可逆重组。然后探索了多步骤组装途径,以提高产量。体素通过柔性链中间体组装成刚性结构,产量提高了 100 倍。我们设想,DNA 折纸体块的可折叠链可以增加可重构纳米材料的复杂性,为纳米机器人系统的组装提供模块化组件,未来可应用于合成生物学、无机材料组装和纳米医学。
Reconfigurable nanomaterials folded from multicomponent chains of DNA origami voxels
In cells, proteins rapidly self-assemble into sophisticated nanomachines. Bioinspired self-assembly approaches, such as DNA origami, have been used to achieve complex three-dimensional (3D) nanostructures and devices. However, current synthetic systems are limited by low yields in hierarchical assembly and challenges in rapid and efficient reconfiguration between diverse structures. Here, we developed a modular system of DNA origami “voxels” with programmable 3D connections. We demonstrate multifunctional pools of up to 12 unique voxels that can assemble into many shapes, prototyping 50 structures. Programmable switching of local connections between flexible and rigid states achieved rapid and reversible reconfiguration of global structures in three dimensions. Multistep assembly pathways were then explored to increase the yield. Voxels were assembled via flexible chain intermediates into rigid structures, increasing yield up to 100-fold. We envision that foldable chains of DNA origami voxels can achieve increased complexity in reconfigurable nanomaterials, providing modular components for the assembly of nanorobotic systems with future applications in synthetic biology, assembly of inorganic materials, and nanomedicine.
期刊介绍:
Science Robotics publishes original, peer-reviewed, science- or engineering-based research articles that advance the field of robotics. The journal also features editor-commissioned Reviews. An international team of academic editors holds Science Robotics articles to the same high-quality standard that is the hallmark of the Science family of journals.
Sub-topics include: actuators, advanced materials, artificial Intelligence, autonomous vehicles, bio-inspired design, exoskeletons, fabrication, field robotics, human-robot interaction, humanoids, industrial robotics, kinematics, machine learning, material science, medical technology, motion planning and control, micro- and nano-robotics, multi-robot control, sensors, service robotics, social and ethical issues, soft robotics, and space, planetary and undersea exploration.