{"title":"皮损中 HIF-1α 和 miR-199a 水平与银屑病严重程度指数的相关性分析。","authors":"Gang Miao, Jing Pan, Lingyan Wang, Fenfen Li","doi":"10.5114/ada.2024.143495","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The pathogenesis of psoriasis is complex. Previous bioinformatics studies have found differential expression of HIF-1α and miR-199 in psoriasis, but the correlation between miR-199a and HIF-1α and the severity of psoriasis is still unclear. This study found differential expression of HIF-1α and miR-199a levels in the skin lesions of patients with different degrees of psoriasis. HIF-1α mRNA and miR-199a are PASI influencing factors, and the levels of HIF-1 α and miR-199a in the skin lesions can serve as potential indicators for evaluating the severity index of psoriasis.</p><p><strong>Aim: </strong>To examine the relationships between the levels of HIF-1α and miR-199a and psoriasis severity.</p><p><strong>Material and methods: </strong>Sixty-five patients with psoriasis vulgaris treated from January 2023 to May 2024 were divided into a mild group (<i>n</i> = 16), a moderate group (<i>n</i> = 23), and a severe group (<i>n</i> = 26) according to the lesion area and severity index (PASI) score, and 40 healthy people were included in the control group (group A). The expression of HIF-1a and miR-199a in psoriatic lesions and normal skin tissues was detected by fluorescence quantitative PCR.</p><p><strong>Results: </strong>The level of miR-199a in lesions in the observation group (group B) was lower than that in group A, but the level of HIF-1αmRNA in group B was greater than that in group A. The level of miR-199a in moderate and severe psoriasis patients was lower than that in mild psoriasis patients, and that in severe psoriasis patients was lower than that in moderate psoriasis patients. HIF-1α mRNA in lesions was positively associated with the PASI in psoriasis patients, and the miR-199a level in lesions was positively associated with the PASI in psoriasis patients. Furthermore, taking the PASI as the dependent variable and HIF-1αmRNA and miR-199a with a linear relationship with the dependent variable as the independent variables, multiple stepwise regression analysis showed that HIF-1α mRNA and miR-199a influenced the PASI.</p><p><strong>Conclusions: </strong>HIF-1α and miR-199a are differentially expressed in the lesions of patients with different severities of psoriasis. The HIF-1α and miR-199a levels in lesions can be used as potential indices to evaluate the severity of psoriasis.</p>","PeriodicalId":54595,"journal":{"name":"Postepy Dermatologii I Alergologii","volume":"41 5","pages":"521-524"},"PeriodicalIF":1.4000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589632/pdf/","citationCount":"0","resultStr":"{\"title\":\"Analysis of the correlation between the levels of HIF-1α and miR-199a in lesions and the psoriasis severity index.\",\"authors\":\"Gang Miao, Jing Pan, Lingyan Wang, Fenfen Li\",\"doi\":\"10.5114/ada.2024.143495\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>The pathogenesis of psoriasis is complex. Previous bioinformatics studies have found differential expression of HIF-1α and miR-199 in psoriasis, but the correlation between miR-199a and HIF-1α and the severity of psoriasis is still unclear. This study found differential expression of HIF-1α and miR-199a levels in the skin lesions of patients with different degrees of psoriasis. HIF-1α mRNA and miR-199a are PASI influencing factors, and the levels of HIF-1 α and miR-199a in the skin lesions can serve as potential indicators for evaluating the severity index of psoriasis.</p><p><strong>Aim: </strong>To examine the relationships between the levels of HIF-1α and miR-199a and psoriasis severity.</p><p><strong>Material and methods: </strong>Sixty-five patients with psoriasis vulgaris treated from January 2023 to May 2024 were divided into a mild group (<i>n</i> = 16), a moderate group (<i>n</i> = 23), and a severe group (<i>n</i> = 26) according to the lesion area and severity index (PASI) score, and 40 healthy people were included in the control group (group A). The expression of HIF-1a and miR-199a in psoriatic lesions and normal skin tissues was detected by fluorescence quantitative PCR.</p><p><strong>Results: </strong>The level of miR-199a in lesions in the observation group (group B) was lower than that in group A, but the level of HIF-1αmRNA in group B was greater than that in group A. The level of miR-199a in moderate and severe psoriasis patients was lower than that in mild psoriasis patients, and that in severe psoriasis patients was lower than that in moderate psoriasis patients. HIF-1α mRNA in lesions was positively associated with the PASI in psoriasis patients, and the miR-199a level in lesions was positively associated with the PASI in psoriasis patients. Furthermore, taking the PASI as the dependent variable and HIF-1αmRNA and miR-199a with a linear relationship with the dependent variable as the independent variables, multiple stepwise regression analysis showed that HIF-1α mRNA and miR-199a influenced the PASI.</p><p><strong>Conclusions: </strong>HIF-1α and miR-199a are differentially expressed in the lesions of patients with different severities of psoriasis. The HIF-1α and miR-199a levels in lesions can be used as potential indices to evaluate the severity of psoriasis.</p>\",\"PeriodicalId\":54595,\"journal\":{\"name\":\"Postepy Dermatologii I Alergologii\",\"volume\":\"41 5\",\"pages\":\"521-524\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589632/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Postepy Dermatologii I Alergologii\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.5114/ada.2024.143495\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/9/10 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ALLERGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Postepy Dermatologii I Alergologii","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.5114/ada.2024.143495","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/10 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ALLERGY","Score":null,"Total":0}
引用次数: 0
摘要
简介银屑病的发病机制十分复杂。以往的生物信息学研究发现,HIF-1α和miR-199在银屑病中有差异表达,但miR-199a和HIF-1α与银屑病严重程度的相关性仍不清楚。本研究发现,在不同程度的银屑病患者皮损中,HIF-1α和miR-199a的表达水平存在差异。HIF-1α mRNA和miR-199a是PASI的影响因素,皮损中HIF-1 α和miR-199a的水平可作为评价银屑病严重程度指数的潜在指标:根据皮损面积和严重程度指数(PASI)评分将2023年1月至2024年5月接受治疗的65名寻常型银屑病患者分为轻度组(16人)、中度组(23人)和重度组(26人),并将40名健康人纳入对照组(A组)。荧光定量 PCR 检测银屑病皮损和正常皮肤组织中 HIF-1a 和 miR-199a 的表达:结果:观察组(B 组)皮损中 miR-199a 的水平低于 A 组,但 B 组 HIF-1αmRNA 的水平高于 A 组。皮损中的 HIF-1α mRNA 与银屑病患者的 PASI 呈正相关,皮损中的 miR-199a 水平与银屑病患者的 PASI 呈正相关。此外,以PASI为因变量,以与因变量呈线性关系的HIF-1αmRNA和miR-199a为自变量,多元逐步回归分析表明,HIF-1αmRNA和miR-199a对PASI有影响:结论:HIF-1α和miR-199a在不同严重程度银屑病患者的皮损中表达不同。皮损中的HIF-1α和miR-199a水平可作为评估银屑病严重程度的潜在指标。
Analysis of the correlation between the levels of HIF-1α and miR-199a in lesions and the psoriasis severity index.
Introduction: The pathogenesis of psoriasis is complex. Previous bioinformatics studies have found differential expression of HIF-1α and miR-199 in psoriasis, but the correlation between miR-199a and HIF-1α and the severity of psoriasis is still unclear. This study found differential expression of HIF-1α and miR-199a levels in the skin lesions of patients with different degrees of psoriasis. HIF-1α mRNA and miR-199a are PASI influencing factors, and the levels of HIF-1 α and miR-199a in the skin lesions can serve as potential indicators for evaluating the severity index of psoriasis.
Aim: To examine the relationships between the levels of HIF-1α and miR-199a and psoriasis severity.
Material and methods: Sixty-five patients with psoriasis vulgaris treated from January 2023 to May 2024 were divided into a mild group (n = 16), a moderate group (n = 23), and a severe group (n = 26) according to the lesion area and severity index (PASI) score, and 40 healthy people were included in the control group (group A). The expression of HIF-1a and miR-199a in psoriatic lesions and normal skin tissues was detected by fluorescence quantitative PCR.
Results: The level of miR-199a in lesions in the observation group (group B) was lower than that in group A, but the level of HIF-1αmRNA in group B was greater than that in group A. The level of miR-199a in moderate and severe psoriasis patients was lower than that in mild psoriasis patients, and that in severe psoriasis patients was lower than that in moderate psoriasis patients. HIF-1α mRNA in lesions was positively associated with the PASI in psoriasis patients, and the miR-199a level in lesions was positively associated with the PASI in psoriasis patients. Furthermore, taking the PASI as the dependent variable and HIF-1αmRNA and miR-199a with a linear relationship with the dependent variable as the independent variables, multiple stepwise regression analysis showed that HIF-1α mRNA and miR-199a influenced the PASI.
Conclusions: HIF-1α and miR-199a are differentially expressed in the lesions of patients with different severities of psoriasis. The HIF-1α and miR-199a levels in lesions can be used as potential indices to evaluate the severity of psoriasis.