[稀土氧化钐尘肺病工人血清蛋白质组特征研究]。

Q3 Medicine
X H Wang, R X Ding, T Ma, Y N Bi, H J Yin
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引用次数: 0

摘要

目的筛选稀土氧化钐尘肺病工人血清中的差异蛋白,为寻找其早期诊断生物标志物提供新思路。研究方法2019年4月,选取内蒙古自治区包头市某稀土厂3名确诊为氧化钐尘肺病的男工为观察组,3名未接触粉尘的男工为对照组。采用无标记蛋白质组学方法对血清进行测序,筛选差异表达蛋白质,然后进行蛋白质同源群(COG)注释、基因本体(GO)富集分析和京都基因和基因组百科全书(KEGG)通路分析。使用相互作用基因库检索工具数据库和 Cytoscape 3.9.1 软件绘制蛋白质-蛋白质相互作用网络。使用CytoHubba插件筛选差异表达的高分蛋白质,并使用实时荧光定量聚合酶链反应(RT/q-PCR)验证蛋白质组测序结果。结果显示共筛选出稀土氧化钐尘肺病患者血清中 45 个上调差异表达蛋白和 5 个下调差异表达蛋白。在 COG 功能分类中,翻译后修饰、蛋白质周转和伴侣蛋白的数量最多。GO 富集包括 25 个生物过程条目,如补体激活(经典途径);15 个细胞成分条目,如细胞外重组物;10 个分子功能条目,如蛋白质结合。通过 KEGG 富集分析确定的通路主要包括传染病、免疫系统、信号转导和免疫相关疾病。得分前 10 位的蛋白质分别是血红蛋白、补体 C1r 亚组分、补体 C1s 亚组分、载脂蛋白 C-Ⅲ、载脂蛋白 A-Ⅱ、凝血酶原、阿法明、补体成分 C8 γ 链、补体成分 C6、补体成分 C7。RT/q-PCR 验证结果显示,观察组血红蛋白、凝血酶原和补体 C1s 亚组分的 mRNA 表达水平明显高于对照组,差异有统计学意义(PConclusion:筛选出稀土氧化钐尘肺病工人血清中10种差异表达蛋白,为筛选氧化钐尘肺病早期诊断的生物标志物提供了一个很好的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Study on serum proteomic characteristics of workers with rare earth samarium oxide pneumoconiosis].

Objective: To screen differential proteins in the serum of workers with rare earth samarium oxide pneumoconiosis, in order to provide new ideas for finding its early diagnostic biomarkers. Methods: In April 2019, three male workers diagnosed with samarium oxide pneumoconiosis at a rare earth factory in Baotou City, Inner Mongolia Autonomous Region were selected as the observation group, and three male workers who were not exposed to dust were selected as the control group. The serum was sequenced using the Label-free proteomic method to screen for differentially expressed proteins, followed by cluster of orthologous groups of proteins (COG) annotation, gene ontology (GO) enrichment analysis, and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis. The interaction gene library retrieval tool database and Cytoscape 3.9.1 software were used to draw protein-protein interaction networks. CytoHubba plugin was used to screen for differentially expressed proteins with high scores, and real-time fluorescence quantitative polymerase chain reaction (RT/q-PCR) was used to validate the proteomic sequencing results. Results: A total of 45 up-regulated differentially expressed proteins and 5 down-regulated differentially expressed proteins were screened out in the serum of workers with rare earth samarium oxide pneumoconiosis. In the COG functional classification, post-translational modifications, protein turnover, and chaperones were the most numerous. GO enrichment included 25 entries for biological processes such as complement activation (classical pathways), 15 entries for cellular components such as extracellular recombinants, and 10 entries for molecular functions such as protein binding. The pathways identified by KEGG enrichment analysis mainly included infectious diseases, immune system, signal transduction, and immune related diseases. The top 10 scoring proteins were haptoglobin, complement C1r subcomponent, complement C1s subcomponent, apolipoprotein C-Ⅲ, apolipoprotein A-Ⅱ, prothrombin, afamin, complement component C8 gamma chain, complement component C6, complement component C7. The RT/q-PCR validation results showed that the mRNA expression levels of haptoglobin, prothrombin and complement C1s subcomponent in the observation group were significantly higher than those in the control group, and the differences were statistically significant (P<0.05) . Conclusion: Ten differentially expressed proteins in the serum of workers with rare earth samarium oxide pneumoconiosis are screened, which provides a good idea for the screening of biomarkers for early diagnosis of samarium oxide pneumoconiosis.

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中华劳动卫生职业病杂志
中华劳动卫生职业病杂志 Medicine-Medicine (all)
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