淀粉芽孢杆菌 SS-CR10 在辣椒上对 Agroathelia rolfsii 的多方面生长促进和生物防治作用以及诱导防御机制。

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Chandagalu Ramesh Santhosh, Shivannegowda Mahadevakumar, Bettadapura Rameshgowda Nuthan, Madeva Bharatha, Javaraiah Parashiva, Madappa Mahesh, Siddaiah Chandranayaka, Sreedharamurthy Satish
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引用次数: 0

摘要

植物生长促进(PGP)内生细菌是一种有益微生物,可帮助植物抵御真菌植物病原体造成的生物胁迫。本研究从辣椒(Capsicum annuum L.)中分离出 78 个内生细菌。利用 16S rDNA 同源性筛选出一种具有多种 PGP 属性且对 Agroathelia rolfsii 有较好抑制作用的强效分离物,并确定其为淀粉芽孢杆菌。SS_CR10 菌株具有磷酸盐溶解(65.9 μg ml-1)、固氮、产生 IAA(9.77 至 24.45 μg ml-1)、氨、ACC 脱氨酶、苷元和水解酶等 PGP 特性。此外,该菌株还表现出了在根部定殖的能力。它明显改善了植物的发育性状,如鲜重和干重、光合色素、根和芽的长度。脂肽基因的 LC-MS 分析和 PCR 扩增也表明,其中存在表面活性素、iturin 和杆菌素脂肽。用脂肽提取物和细菌悬浮液处理后,通过荧光显微镜和扫描电子显微镜(SEM)的活死人染色,可以看到 A. rolfsii 菌丝体出现了严重的变形和细胞死亡。最后,细菌处理后防御相关基因 CaPR1、CaPR2 和 CaPR4 的上调证实了系统抗性的诱导。总之,本研究表明 SS_CR10 菌株可能有助于促进辣椒中植物的生长,并以生态友好的方式控制根瘤蚜,从而保护土壤健康。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multifaceted growth promotion and biocontrol of Agroathelia rolfsii and induction of defense mechanism by Bacillus amyloliquefaciens SS-CR10 on chilli.

Plant-growth-promoting (PGP) endophytic bacteria are beneficial microorganisms that can help plants withstand biotic stress caused by fungal phytopathogens. In the present study, 78 endophytic bacterial isolates were isolated from chilli (Capsicum annuum L.). A potent isolate with several PGP attributes and better inhibition against Agroathelia rolfsii was selected and identified as Bacillus amyloliquefaciens using 16S rDNA homologies. Phosphate solubilization (65.9 μg ml-1), nitrogen fixation, production of IAA (9.77 to 24.45 μg ml-1), ammonia, ACC deaminase, siderophore, and hydrolytic enzymes were among the PGP traits shown by the strain SS_CR10. Furthermore, the strain demonstrated the ability to colonize roots. It significantly improved the plant's developmental traits, such as fresh and dry weight, photosynthetic pigments, and root and shoot length. LC-MS analysis and PCR amplification of lipopeptide genes also showed that surfactin, iturin, and bacilysin lipopeptides were present. Following treatments with lipopeptide extract and bacterial suspension, A. rolfsii mycelia showed severe deformation and cell death, as seen by live dead staining by fluorescent microscopy and scanning electron microscopy (SEM). Finally, the upregulation of defense-related genes CaPR1, CaPR2, and CaPR4 after bacterial treatment confirmed the induction of systemic resistance. In conclusion, this study shows how strain SS_CR10 might be useful for promoting plant growth in chilli and controlling A. rolfsii in an eco-friendly way, which would protect the health of the soil.

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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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