西瓜(Citrullus lanatus)中 JAZ 基因的全基因组调查和表达分析。

IF 2.6 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yan-Ge Li, Jing Zhang, Xiu-Xiu Cai, Le-Ping Fan, Zhong-Hou Zhu, Xue-Jie Zhu, Da-Long Guo
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引用次数: 0

摘要

背景 JAZ:(Jasmonate ZIM-domain)基因在植物生长和 JA 信号通路中发挥重要作用,而 JA 信号通路与果实成熟过程相关。然而,有关西瓜中 JAZ 基因的全基因组鉴定及其与果实成熟关系的报道很少。方法与结果:本研究采用生物信息学方法在全基因组水平鉴定了西瓜中的 ClaJAZ 基因。进一步探讨了 JAZ 基因的系统发育关系、染色体映射、启动子动态、表达和结构特征。结果表明,在西瓜基因组中共发现了9个ClaJAZ基因,它们不均匀地分布在6条染色体上,并且都具有保守的Jas和TIFY结构域。这些 JAZ 基因被分为四个不同的组,其中五个基因涉及染色体间的串联重复事件,同一亚组的成员在基因结构和蛋白质基序模式上表现出高度的相似性。对 ClaJAZ 基因启动子区域的分析表明,存在与激素反应、压力和发育过程相关的顺式作用元件。通过实时定量 PCR(qRT-PCR)进行的基因表达分析表明,在果实发育的不同阶段,ClaJAZ 基因的表达存在时空差异。其中,ClaJAZ7 的转录表达水平最高,并表现出较强的启动子活性。结论:本研究对 ClaJAZ 基因进行了全面分析,深入了解了 ClaJAZ 在西瓜果实发育和成熟过程中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome-wide survey and expression analysis of JAZ genes in watermelon (Citrullus lanatus).

BACKGROUND JAZ: (Jasmonate ZIM-domain) genes play important roles in plant growth and JA signaling pathway which is correlated with fruit ripening process. However, there have been few reports on the genome-wide identification of JAZ genes in watermelon and its relationship with fruit ripening. METHODS AND RESULTS: In this study, bioinformatics approaches were employed to identify ClaJAZ genes of watermelon at the genome-wide levels. Further exploration delved into the phylogenetic relationships, chromosomal mappings, promoter dynamics, expression, and architectural features of the JAZ genes. The results showed that a total of 9 ClaJAZ genes unevenly distributed across six chromosomes were identified in the watermelon genome, and they all have conserved Jas and TIFY domains. These JAZ genes were divided into four distinct groups with five genes involved in inter-chromosomal tandem duplication events, and members of the same subgroup exhibited a high degree of similarity in their gene structure and protein motif patterns. Analysis of the promoter regions of the ClaJAZ genes indicated the presence of cis-acting elements associated with hormonal responses, stress, and developmental processes. Gene expression analysis through real-time quantitative PCR (qRT-PCR) showed that there were spatiotemporal differences in the expression of ClaJAZ genes at various stages of fruit development. Among them, ClaJAZ7 has the highest level of transcriptional expression and showed strong promoter activity. CONCLUSIONS: This study conducted a comprehensive analysis of the ClaJAZ genes and provided insights into the role of ClaJAZ in the development and ripening of watermelon fruit.

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来源期刊
Molecular Biology Reports
Molecular Biology Reports 生物-生化与分子生物学
CiteScore
5.00
自引率
0.00%
发文量
1048
审稿时长
5.6 months
期刊介绍: Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews.
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