{"title":"益生菌植物乳杆菌通过调节肠道微生物菌群激活 PPARγ 并抑制 MAPKs/NF-κB 来缓解结肠炎。","authors":"Rongxin Zang, Rui Zhou, Yaodong Li, Huihao Wu, Liping Lu, Hongwei Xu","doi":"10.1007/s00394-024-03520-w","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>In order to address the global public health concern of colitis, this study was conducted to investigate the beneficial effect of Lactobacillus plantarum LR002 (LR) on the remission of ulcerative colitis (UC) in mice and to explore the possible underlying mechanisms.</p><p><strong>Methods: </strong>The effect of LR on UC was analyzed by using dextran sodium sulfate (DSS)-induced UC model in mice (n = 9). To assess the therapeutic effect of LR on UC in mice, the disease activity index (DAI) of mice, histopathological alterations, intestinal epithelial barrier integrity, and intestinal microflora were determined.</p><p><strong>Results: </strong>The results demonstrated a reduction in the DSS-induced DAI in UC mice. Additionally, it mitigated colon shortening, minimized intestinal tissue damage, and preserved intestinal tight junction proteins (Claudin-3, Occludin, and ZO-1). LR reduced the levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and oxidative mediators (MPO, SOD and MDA) in the colon of UC mice, which could also alleviate the imbalance of intestinal flora in UC mice, increase the abundance of Prevotellaceae, and Ligilactobacillus, and decrease the abundance of Bacteroidaceae and Eubacteriumrum. LR can also increase the levels of PPARγ in the nucleus and inhibit the MAPK/NF-ĸB signaling pathway in UC mice. Besides, the reduction of the content of short-chain fatty acid (SCFAs) in the colon of UC mice was relieved.</p><p><strong>Conclusion: </strong>The above results forge a scientific basis for LR as natural anti-inflammatory food to improve the imbalance of inflammatory intestinal flora and promote intestinal health.</p>","PeriodicalId":12030,"journal":{"name":"European Journal of Nutrition","volume":"64 1","pages":"32"},"PeriodicalIF":4.1000,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The probiotic Lactobacillus plantarum alleviates colitis by modulating gut microflora to activate PPARγ and inhibit MAPKs/NF-κB.\",\"authors\":\"Rongxin Zang, Rui Zhou, Yaodong Li, Huihao Wu, Liping Lu, Hongwei Xu\",\"doi\":\"10.1007/s00394-024-03520-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>In order to address the global public health concern of colitis, this study was conducted to investigate the beneficial effect of Lactobacillus plantarum LR002 (LR) on the remission of ulcerative colitis (UC) in mice and to explore the possible underlying mechanisms.</p><p><strong>Methods: </strong>The effect of LR on UC was analyzed by using dextran sodium sulfate (DSS)-induced UC model in mice (n = 9). To assess the therapeutic effect of LR on UC in mice, the disease activity index (DAI) of mice, histopathological alterations, intestinal epithelial barrier integrity, and intestinal microflora were determined.</p><p><strong>Results: </strong>The results demonstrated a reduction in the DSS-induced DAI in UC mice. Additionally, it mitigated colon shortening, minimized intestinal tissue damage, and preserved intestinal tight junction proteins (Claudin-3, Occludin, and ZO-1). LR reduced the levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and oxidative mediators (MPO, SOD and MDA) in the colon of UC mice, which could also alleviate the imbalance of intestinal flora in UC mice, increase the abundance of Prevotellaceae, and Ligilactobacillus, and decrease the abundance of Bacteroidaceae and Eubacteriumrum. LR can also increase the levels of PPARγ in the nucleus and inhibit the MAPK/NF-ĸB signaling pathway in UC mice. Besides, the reduction of the content of short-chain fatty acid (SCFAs) in the colon of UC mice was relieved.</p><p><strong>Conclusion: </strong>The above results forge a scientific basis for LR as natural anti-inflammatory food to improve the imbalance of inflammatory intestinal flora and promote intestinal health.</p>\",\"PeriodicalId\":12030,\"journal\":{\"name\":\"European Journal of Nutrition\",\"volume\":\"64 1\",\"pages\":\"32\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Journal of Nutrition\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s00394-024-03520-w\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Nutrition","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00394-024-03520-w","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
The probiotic Lactobacillus plantarum alleviates colitis by modulating gut microflora to activate PPARγ and inhibit MAPKs/NF-κB.
Purpose: In order to address the global public health concern of colitis, this study was conducted to investigate the beneficial effect of Lactobacillus plantarum LR002 (LR) on the remission of ulcerative colitis (UC) in mice and to explore the possible underlying mechanisms.
Methods: The effect of LR on UC was analyzed by using dextran sodium sulfate (DSS)-induced UC model in mice (n = 9). To assess the therapeutic effect of LR on UC in mice, the disease activity index (DAI) of mice, histopathological alterations, intestinal epithelial barrier integrity, and intestinal microflora were determined.
Results: The results demonstrated a reduction in the DSS-induced DAI in UC mice. Additionally, it mitigated colon shortening, minimized intestinal tissue damage, and preserved intestinal tight junction proteins (Claudin-3, Occludin, and ZO-1). LR reduced the levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and oxidative mediators (MPO, SOD and MDA) in the colon of UC mice, which could also alleviate the imbalance of intestinal flora in UC mice, increase the abundance of Prevotellaceae, and Ligilactobacillus, and decrease the abundance of Bacteroidaceae and Eubacteriumrum. LR can also increase the levels of PPARγ in the nucleus and inhibit the MAPK/NF-ĸB signaling pathway in UC mice. Besides, the reduction of the content of short-chain fatty acid (SCFAs) in the colon of UC mice was relieved.
Conclusion: The above results forge a scientific basis for LR as natural anti-inflammatory food to improve the imbalance of inflammatory intestinal flora and promote intestinal health.
期刊介绍:
The European Journal of Nutrition publishes original papers, reviews, and short communications in the nutritional sciences. The manuscripts submitted to the European Journal of Nutrition should have their major focus on the impact of nutrients and non-nutrients on
immunology and inflammation,
gene expression,
metabolism,
chronic diseases, or
carcinogenesis,
or a major focus on
epidemiology, including intervention studies with healthy subjects and with patients,
biofunctionality of food and food components, or
the impact of diet on the environment.