Hannah Lithgow, Laura Gibson, Russell Wilson, Neil Guthrie, Lesley Ingram-Sills, Tom Clifford, Mark Ross
{"title":"在赛后恢复的 48 小时内，超耐力比赛会导致循环调节性 T 细胞、CD4+ 天真 T 细胞和 CD8+ 效应记忆 T 细胞发生变化。","authors":"Hannah Lithgow, Laura Gibson, Russell Wilson, Neil Guthrie, Lesley Ingram-Sills, Tom Clifford, Mark Ross","doi":"10.1007/s00421-024-05677-y","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Exercise is known to acutely affect T-lymphocyte populations in the peripheral blood, which is intensity- and duration-dependent. However, effects of longer duration endurance exercise (>5 h) on T-cells in the days following are unknown. The aim of this study was to investigate the circulating T-cell changes that occur in response to an ultra-endurance event, which may provide insight into the inflammatory response to ultra-endurance exercise.</p><p><strong>Methods: </strong>Ten individuals (m = 7, f = 3) completing an Ironman 70.3 event volunteered for the study. Peripheral blood samples were taken 1-2 days pre-race (PRE-RACE), and 1 day (RACE + 1) and 2 days (RACE + 2) post-race, with circulating T-cells enumerated by flow cytometry (total CD3+, CD4+ and CD8+ T-cells, regulatory T-cells [CD4+CD25+CD127-; T<sub>REG</sub>], naïve [CD27+CD45RA+; NA], central memory [CD27+CD45RA-; CM], effector memory [CD27-CD45RA-; EM], and effector memory CD45RA+ [CD27-CD45RA+; EMRA]).</p><p><strong>Results: </strong>There were no changes in total CD3+, CD4+ and CD8+ T-cells. T<sub>REG</sub> RACE + 1 was significantly higher compared to PRE-RACE, as were the proportion of CD4+ NA cells and CD8+ CM cells at RACE + 2; CD8+ EM cells fell at RACE + 2 (absolute counts and proportion).</p><p><strong>Conclusion: </strong>In conclusion, the ultra-endurance event evoked T-cell changes over the 48 h recovery period, with an increase in T-cells that regulate the immune response, and a reduction in circulating EM T-cells, most likely trafficked to sites of tissue damage and inflammation.</p>","PeriodicalId":12005,"journal":{"name":"European Journal of Applied Physiology","volume":" ","pages":"1129-1138"},"PeriodicalIF":2.8000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950060/pdf/","citationCount":"0","resultStr":"{\"title\":\"An ultra-endurance event leads to changes in circulating regulatory T-cells, CD4+ naïve and CD8+ effector memory T-cells in the 48 h post-race recovery period.\",\"authors\":\"Hannah Lithgow, Laura Gibson, Russell Wilson, Neil Guthrie, Lesley Ingram-Sills, Tom Clifford, Mark Ross\",\"doi\":\"10.1007/s00421-024-05677-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Exercise is known to acutely affect T-lymphocyte populations in the peripheral blood, which is intensity- and duration-dependent. However, effects of longer duration endurance exercise (>5 h) on T-cells in the days following are unknown. The aim of this study was to investigate the circulating T-cell changes that occur in response to an ultra-endurance event, which may provide insight into the inflammatory response to ultra-endurance exercise.</p><p><strong>Methods: </strong>Ten individuals (m = 7, f = 3) completing an Ironman 70.3 event volunteered for the study. Peripheral blood samples were taken 1-2 days pre-race (PRE-RACE), and 1 day (RACE + 1) and 2 days (RACE + 2) post-race, with circulating T-cells enumerated by flow cytometry (total CD3+, CD4+ and CD8+ T-cells, regulatory T-cells [CD4+CD25+CD127-; T<sub>REG</sub>], naïve [CD27+CD45RA+; NA], central memory [CD27+CD45RA-; CM], effector memory [CD27-CD45RA-; EM], and effector memory CD45RA+ [CD27-CD45RA+; EMRA]).</p><p><strong>Results: </strong>There were no changes in total CD3+, CD4+ and CD8+ T-cells. T<sub>REG</sub> RACE + 1 was significantly higher compared to PRE-RACE, as were the proportion of CD4+ NA cells and CD8+ CM cells at RACE + 2; CD8+ EM cells fell at RACE + 2 (absolute counts and proportion).</p><p><strong>Conclusion: </strong>In conclusion, the ultra-endurance event evoked T-cell changes over the 48 h recovery period, with an increase in T-cells that regulate the immune response, and a reduction in circulating EM T-cells, most likely trafficked to sites of tissue damage and inflammation.</p>\",\"PeriodicalId\":12005,\"journal\":{\"name\":\"European Journal of Applied Physiology\",\"volume\":\" \",\"pages\":\"1129-1138\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950060/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Journal of Applied Physiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s00421-024-05677-y\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/11/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"PHYSIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Applied Physiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00421-024-05677-y","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/27 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的：众所周知，运动会对外周血中的 T 淋巴细胞群产生急性影响，这种影响与运动强度和持续时间有关。然而，持续时间较长的耐力运动（>5 小时）对运动后几天内 T 细胞的影响尚不清楚。本研究的目的是调查超耐力运动后循环 T 细胞的变化，从而了解超耐力运动的炎症反应：十名完成铁人三项 70.3 赛程的运动员（男 = 7，女 = 3）自愿参与研究。在赛前 1-2 天（PRE-RACE）、赛后 1 天（RACE + 1）和 2 天（RACE + 2）采集外周血样本，用流式细胞术计算循环 T 细胞（CD3+、CD4+ 和 CD8+ T 细胞总数，调节性 T 细胞 [CD4+CD25+CD127-；TREG]、幼稚T细胞[CD27+CD45RA+；NA]、中心记忆T细胞[CD27+CD45RA-；CM]、效应记忆T细胞[CD27-CD45RA-；EM]和效应记忆CD45RA+[CD27-CD45RA+；EMRA]）。结果：CD3+、CD4+和CD8+ T细胞总数没有变化。TREG RACE + 1与PRE-RACE相比明显升高，RACE + 2时CD4+ NA细胞和CD8+ CM细胞的比例也明显升高；CD8+ EM细胞在RACE + 2时有所下降（绝对计数和比例）：总之，超耐力比赛诱发 T 细胞在 48 小时恢复期内发生变化，调节免疫反应的 T 细胞增加，而循环中的 EM T 细胞减少，这些细胞很可能被输送到组织损伤和炎症部位。
An ultra-endurance event leads to changes in circulating regulatory T-cells, CD4+ naïve and CD8+ effector memory T-cells in the 48 h post-race recovery period.
Purpose: Exercise is known to acutely affect T-lymphocyte populations in the peripheral blood, which is intensity- and duration-dependent. However, effects of longer duration endurance exercise (>5 h) on T-cells in the days following are unknown. The aim of this study was to investigate the circulating T-cell changes that occur in response to an ultra-endurance event, which may provide insight into the inflammatory response to ultra-endurance exercise.
Methods: Ten individuals (m = 7, f = 3) completing an Ironman 70.3 event volunteered for the study. Peripheral blood samples were taken 1-2 days pre-race (PRE-RACE), and 1 day (RACE + 1) and 2 days (RACE + 2) post-race, with circulating T-cells enumerated by flow cytometry (total CD3+, CD4+ and CD8+ T-cells, regulatory T-cells [CD4+CD25+CD127-; TREG], naïve [CD27+CD45RA+; NA], central memory [CD27+CD45RA-; CM], effector memory [CD27-CD45RA-; EM], and effector memory CD45RA+ [CD27-CD45RA+; EMRA]).
Results: There were no changes in total CD3+, CD4+ and CD8+ T-cells. TREG RACE + 1 was significantly higher compared to PRE-RACE, as were the proportion of CD4+ NA cells and CD8+ CM cells at RACE + 2; CD8+ EM cells fell at RACE + 2 (absolute counts and proportion).
Conclusion: In conclusion, the ultra-endurance event evoked T-cell changes over the 48 h recovery period, with an increase in T-cells that regulate the immune response, and a reduction in circulating EM T-cells, most likely trafficked to sites of tissue damage and inflammation.
期刊介绍：
The European Journal of Applied Physiology (EJAP) aims to promote mechanistic advances in human integrative and translational physiology. Physiology is viewed broadly, having overlapping context with related disciplines such as biomechanics, biochemistry, endocrinology, ergonomics, immunology, motor control, and nutrition. EJAP welcomes studies dealing with physical exercise, training and performance. Studies addressing physiological mechanisms are preferred over descriptive studies. Papers dealing with animal models or pathophysiological conditions are not excluded from consideration, but must be clearly relevant to human physiology.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
