J Martin de Los Santos-Ramirez, Vania G Martinez-Gonzalez, Carlos A Mendiola-Escobedo, Jose M Cotera-Sarabia, Roberto C Gallo-Villanueva, Rodrigo Martinez-Duarte, Victor H Perez-Gonzalez
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引用次数: 0
摘要
这篇论文介绍了基于直流绝缘体的电动(DC-iEK)微流体装置,该装置在 9 V 碱性电池的刺激下捕获直径为 2 微米的荧光聚苯乙烯颗粒。这些装置在流体通道内设有两个三角形绝缘柱。在 18 V 电压下(两节 9 V 电池串联)可以清楚地观察到颗粒捕集,但在单节 9 V 电池下只能观察到间歇性的颗粒捕集。粒子捕集是通过测量单对三角柱之间间隙区域相对荧光强度的增加来确定的。结果表明,在直流 iEK 系统中使用低刺激电压(被认为是超低电压)可能比使用高性能和高通量粒子处理(即浓缩、分离、过滤或隔离)更适合对粒子进行准确和精确的电动表征,因为前者可以分别显示出一个定位非常准确的捕集区域,其中的粒子振荡可以忽略不计。
Short Communication: Ultralow Voltage Electrokinetic Particle Trapping in DC-iEK Devices Using 9 V Alkaline Batteries as Power Supply.
This contribution describes direct current insulator-based electrokinetic (DC-iEK) microfluidic devices stimulated by 9 V alkaline batteries for the trapping of 2-µm diameter fluorescent polystyrene particles. These devices featured two triangular insulating posts within the fluidic channel. Particle trapping was clearly observed at 18 V (two 9 V batteries connected in series), but only intermittent particle trapping was observed with a single 9 V battery. Particle trapping was determined by measuring the increase in relative fluorescence intensity at the gap region between the single pair of triangular posts. Results demonstrate that the use of low stimulating voltages (deemed as ultralow) in DC-iEK systems may be more suitable for accurate and precise electrokinetic characterization of particles-by exhibiting a very well-localized trapping region with negligible particle oscillations therein, respectively-than for high-performance and high-throughput particle manipulation (i.e., concentration, separation, filtering, or isolation).
期刊介绍:
ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.).
Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences.
Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases.
Papers describing the application of standard electrophoretic methods will not be considered.
Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics:
• Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry
• Single cell and subcellular analysis
• Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS)
• Nanoscale/nanopore DNA sequencing (next generation sequencing)
• Micro- and nanoscale sample preparation
• Nanoparticles and cells analyses by dielectrophoresis
• Separation-based analysis using nanoparticles, nanotubes and nanowires.