{"title":"通过 LC/MS 分析作为疾病生物标记物的手性分子综述。","authors":"Cemil Aydoğan, Büşra Beltekin Çakan, Ashraf Ali","doi":"10.1002/bmc.6044","DOIUrl":null,"url":null,"abstract":"<p><p>The chiral compounds may be biomarker candidates in human metabolism, which indicates the health status of humans. There are many applications in LC/MS that show that chiral small molecules are promising biomarkers for human diseases. Both clinical and commercial analyses of chiral metabolites are necessary due to the enantiomeric ratios of chiral molecules in biological samples may show both human health status and diseases. This review provides current and advanced LC/MS techniques for the separation and analysis of chiral molecules as disease biomarkers. In particular, sample preparation and chromatographic analysis of potential chiral biomarkers in biological samples are presented. The preparation and applications of several chiral columns used in enantiomeric separation of chiral metabolites/biomarkers by advanced LC/MS techniques are discussed. The improvement of these analyses will enable both the discovery of new chiral biomarkers and the prognosis of human diseases.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":" ","pages":"e6044"},"PeriodicalIF":1.8000,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Review on the Analysis of Chiral Molecules as Disease Biomarkers by LC/MS.\",\"authors\":\"Cemil Aydoğan, Büşra Beltekin Çakan, Ashraf Ali\",\"doi\":\"10.1002/bmc.6044\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The chiral compounds may be biomarker candidates in human metabolism, which indicates the health status of humans. There are many applications in LC/MS that show that chiral small molecules are promising biomarkers for human diseases. Both clinical and commercial analyses of chiral metabolites are necessary due to the enantiomeric ratios of chiral molecules in biological samples may show both human health status and diseases. This review provides current and advanced LC/MS techniques for the separation and analysis of chiral molecules as disease biomarkers. In particular, sample preparation and chromatographic analysis of potential chiral biomarkers in biological samples are presented. The preparation and applications of several chiral columns used in enantiomeric separation of chiral metabolites/biomarkers by advanced LC/MS techniques are discussed. The improvement of these analyses will enable both the discovery of new chiral biomarkers and the prognosis of human diseases.</p>\",\"PeriodicalId\":8861,\"journal\":{\"name\":\"Biomedical Chromatography\",\"volume\":\" \",\"pages\":\"e6044\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Chromatography\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1002/bmc.6044\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Chromatography","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/bmc.6044","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
A Review on the Analysis of Chiral Molecules as Disease Biomarkers by LC/MS.
The chiral compounds may be biomarker candidates in human metabolism, which indicates the health status of humans. There are many applications in LC/MS that show that chiral small molecules are promising biomarkers for human diseases. Both clinical and commercial analyses of chiral metabolites are necessary due to the enantiomeric ratios of chiral molecules in biological samples may show both human health status and diseases. This review provides current and advanced LC/MS techniques for the separation and analysis of chiral molecules as disease biomarkers. In particular, sample preparation and chromatographic analysis of potential chiral biomarkers in biological samples are presented. The preparation and applications of several chiral columns used in enantiomeric separation of chiral metabolites/biomarkers by advanced LC/MS techniques are discussed. The improvement of these analyses will enable both the discovery of new chiral biomarkers and the prognosis of human diseases.
期刊介绍：
Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
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