Hanaa S Hassan, Mostafa N Feleafel, Mina S R Abd El-Lahot, Mervat El-Hefny, Taghreed F M Abdel Rahman, Abeer A Mohamed, Doaa Y Abd-Elkader, R M Mahdy
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Therefore, in the present study, fertilizing with potassium citrate as a foliar spray and humic acid (HA) as a soil application allowed for the evaluation of vegetative growth parameters (plant height, number of leaves/plant), total phenolic content, total carbohydrate, antioxidant activity, the essential oil (EO) composition, and bulb yield of garlic (Allium sativum L.). These were carried out in two field experiments throughout the 2020-2021 and 2021-2022 growth seasons. A gas chromatography-mass spectroscopy (GC-MS) apparatus was performed to determine the chemical composition of the isolated EOs. The antifungal activity of the EOs was assessed against two fungi, Fusarium proliferatum and Macrophomina phaseolina, that cause geranium plants to wilt and decay. The findings indicated that applying HA at a rate of 2 g/L with potassium citrate at a rate of 5 or 10 mL/L produced garlic bulbs with the highest levels of productivity and diameter. The diverse treatments between HA with potassium citrate resulted in significant variations in the bioactive components, such as total phenol content, antioxidant activity, total carbohydrate, and sulfur content. The analysis of the EOs revealed the presence of dimethyl trisulfide, diallyl disulfide, methyl 2-propenyl trisulfide, allitridin, and methyl allyl disulfide and allyl tetrasulfide as main compounds. By gradually increasing the concentration of the garlic EO to 4000 µg/mL compared to the control, the inhibition percentage of fungal growth of F. proliferatum and M. phaseolina was increased. 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引用次数: 0
摘要
为了养活世界上日益增长的人口,农业部门近来必须在减少对生态系统和人类健康的有害影响与提高资源效率和产量之间取得平衡。实际上,农药和化肥对农业至关重要,是农民在有利和不利条件下提高产量和保证四季稳定生产的有用工具。因此,在本研究中,通过叶面喷施柠檬酸钾和土壤施用腐植酸(HA),对大蒜(Allium sativum L.)的植株生长参数(株高、叶片数/株)、总酚含量、总碳水化合物、抗氧化活性、精油(EO)成分和球茎产量进行了评估。这些研究分别在 2020-2021 年和 2021-2022 年两个生长季节进行。采用气相色谱-质谱仪(GC-MS)测定了分离出的环氧乙烷的化学成分。对 EO 的抗真菌活性进行了评估,主要针对导致天竺葵植物枯萎和腐烂的两种真菌 Fusarium proliferatum 和 Macrophomina phaseolina。研究结果表明,以每升 5 毫升或 10 毫升的比例施用 2 克 HA 和柠檬酸钾,生产出的大蒜鳞茎产量和直径最高。HA与柠檬酸钾之间的不同处理导致生物活性成分(如总酚含量、抗氧化活性、总碳水化合物和硫含量)的显著变化。对环氧乙烷的分析表明,二甲基三硫醚、二烯丙基二硫醚、甲基 2-丙烯基三硫醚、allitridin、甲基烯丙基二硫醚和烯丙基四硫醚是主要的化合物。与对照组相比,将大蒜环氧乙烷的浓度逐渐增加到 4000 µg/mL 时,对 F. proliferatum 和 M. phaseolina 真菌生长的抑制率有所提高。总之,高浓度的 HA 与柠檬酸钾(5 或 10 mL/L)可作为肥料施用,提高大蒜植株的产量和环氧乙烷含量。
Biostimulants for enhancing productivity, bioactive components, and the essential oils of garlic with the potential antifungal activity.
To feed the world's growing population, the agriculture sector has recently had to strike a balance between reducing its detrimental effects on ecosystems and human health and boosting resource efficiency and production. In reality, pesticides and fertilizers are vital to agriculture and are useful instruments that farmers can employ to increase yield and guarantee steady productivity throughout the seasons under both favorable and unfavorable conditions. Therefore, in the present study, fertilizing with potassium citrate as a foliar spray and humic acid (HA) as a soil application allowed for the evaluation of vegetative growth parameters (plant height, number of leaves/plant), total phenolic content, total carbohydrate, antioxidant activity, the essential oil (EO) composition, and bulb yield of garlic (Allium sativum L.). These were carried out in two field experiments throughout the 2020-2021 and 2021-2022 growth seasons. A gas chromatography-mass spectroscopy (GC-MS) apparatus was performed to determine the chemical composition of the isolated EOs. The antifungal activity of the EOs was assessed against two fungi, Fusarium proliferatum and Macrophomina phaseolina, that cause geranium plants to wilt and decay. The findings indicated that applying HA at a rate of 2 g/L with potassium citrate at a rate of 5 or 10 mL/L produced garlic bulbs with the highest levels of productivity and diameter. The diverse treatments between HA with potassium citrate resulted in significant variations in the bioactive components, such as total phenol content, antioxidant activity, total carbohydrate, and sulfur content. The analysis of the EOs revealed the presence of dimethyl trisulfide, diallyl disulfide, methyl 2-propenyl trisulfide, allitridin, and methyl allyl disulfide and allyl tetrasulfide as main compounds. By gradually increasing the concentration of the garlic EO to 4000 µg/mL compared to the control, the inhibition percentage of fungal growth of F. proliferatum and M. phaseolina was increased. In conclusion, a high concentration of HA with potassium citrate (5 or 10 mL/L), may be suitable and highly appreciated as a fertilizer application to enhance the productivity and EOs content of garlic plants.
期刊介绍:
AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.