{"title":"基于 HS-SPME-GC-MS 和 HS-GC-IMS 对 Alpinia katsumadai Hayata 不同部位的成分和抗菌活性的深入研究","authors":"Jiaxuan Chen, Xue Chen, Yang Zhang, Jingyi Xie, Xuejuan Li, Xuehao Sun, Jiaxin Yin, Songtao Bie, Xinbo Song, Heshui Yu, Zheng Li","doi":"10.1002/rcm.9949","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Rationale</h3>\n \n <p>The volatile organic compounds (VOCs) of <i>Alpinia katsumadai</i> Hayata (AKH) play a key role in determining its effects such as organoleptic properties, medicinal properties, and consumer preferences. The nonmedicinal parts (roots, fibrous roots, stems, leaves, and shells) in AKH are also rich in VOCs and different degrees of antibacterial activity. Therefore, it is important to comprehensively characterize the VOCs in different parts of AKH and learn about their potential antimicrobial abilities.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>In this study, headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) and headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS) were used to comprehensively investigate the compositional characteristics and differences of roots, fibrous roots, stems, leaves, shells, and seeds in AKH. Multivariate statistical analysis was used to determine the differential VOCs in different parts of AKH. The inhibition zone diameter method was used to compare the antibacterial ability of different parts.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>HS-GC-IMS and HS-SPME-GC-MS analyses identified 67 and 125 VOCs, respectively. Multivariate statistical analysis showed the differences in the six parts is obvious. Compounds such as (<i>E</i>)-2-heptenal and thymol were selected as potential VOCs to distinguish six sites of AKH. In general, the inhibition effect of the volatile oils from shell and root on the two colonies was better, and the volatile oils of the four parts of AKH have better inhibitory effect on <i>Staphylococcus aureus</i>.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>This research conducted a comprehensive analysis of the fundamental volatile components in AKH. It subsequently elucidated the distinctions and specificities in different parts. The antibacterial effect of volatile oil in different parts was further compared, which was conducive to finding alternative parts for seeds in some active aspects, and the utilization of AKH was more sufficient.</p>\n </section>\n </div>","PeriodicalId":225,"journal":{"name":"Rapid Communications in Mass Spectrometry","volume":"39 3","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Insights Into the Composition and Antibacterial Activity of Different Parts of Alpinia katsumadai Hayata Based on HS-SPME-GC-MS and HS-GC-IMS\",\"authors\":\"Jiaxuan Chen, Xue Chen, Yang Zhang, Jingyi Xie, Xuejuan Li, Xuehao Sun, Jiaxin Yin, Songtao Bie, Xinbo Song, Heshui Yu, Zheng Li\",\"doi\":\"10.1002/rcm.9949\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Rationale</h3>\\n \\n <p>The volatile organic compounds (VOCs) of <i>Alpinia katsumadai</i> Hayata (AKH) play a key role in determining its effects such as organoleptic properties, medicinal properties, and consumer preferences. The nonmedicinal parts (roots, fibrous roots, stems, leaves, and shells) in AKH are also rich in VOCs and different degrees of antibacterial activity. Therefore, it is important to comprehensively characterize the VOCs in different parts of AKH and learn about their potential antimicrobial abilities.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>In this study, headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) and headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS) were used to comprehensively investigate the compositional characteristics and differences of roots, fibrous roots, stems, leaves, shells, and seeds in AKH. Multivariate statistical analysis was used to determine the differential VOCs in different parts of AKH. The inhibition zone diameter method was used to compare the antibacterial ability of different parts.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>HS-GC-IMS and HS-SPME-GC-MS analyses identified 67 and 125 VOCs, respectively. Multivariate statistical analysis showed the differences in the six parts is obvious. Compounds such as (<i>E</i>)-2-heptenal and thymol were selected as potential VOCs to distinguish six sites of AKH. In general, the inhibition effect of the volatile oils from shell and root on the two colonies was better, and the volatile oils of the four parts of AKH have better inhibitory effect on <i>Staphylococcus aureus</i>.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>This research conducted a comprehensive analysis of the fundamental volatile components in AKH. It subsequently elucidated the distinctions and specificities in different parts. The antibacterial effect of volatile oil in different parts was further compared, which was conducive to finding alternative parts for seeds in some active aspects, and the utilization of AKH was more sufficient.</p>\\n </section>\\n </div>\",\"PeriodicalId\":225,\"journal\":{\"name\":\"Rapid Communications in Mass Spectrometry\",\"volume\":\"39 3\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rapid Communications in Mass Spectrometry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/rcm.9949\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rapid Communications in Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/rcm.9949","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Insights Into the Composition and Antibacterial Activity of Different Parts of Alpinia katsumadai Hayata Based on HS-SPME-GC-MS and HS-GC-IMS
Rationale
The volatile organic compounds (VOCs) of Alpinia katsumadai Hayata (AKH) play a key role in determining its effects such as organoleptic properties, medicinal properties, and consumer preferences. The nonmedicinal parts (roots, fibrous roots, stems, leaves, and shells) in AKH are also rich in VOCs and different degrees of antibacterial activity. Therefore, it is important to comprehensively characterize the VOCs in different parts of AKH and learn about their potential antimicrobial abilities.
Methods
In this study, headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) and headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS) were used to comprehensively investigate the compositional characteristics and differences of roots, fibrous roots, stems, leaves, shells, and seeds in AKH. Multivariate statistical analysis was used to determine the differential VOCs in different parts of AKH. The inhibition zone diameter method was used to compare the antibacterial ability of different parts.
Results
HS-GC-IMS and HS-SPME-GC-MS analyses identified 67 and 125 VOCs, respectively. Multivariate statistical analysis showed the differences in the six parts is obvious. Compounds such as (E)-2-heptenal and thymol were selected as potential VOCs to distinguish six sites of AKH. In general, the inhibition effect of the volatile oils from shell and root on the two colonies was better, and the volatile oils of the four parts of AKH have better inhibitory effect on Staphylococcus aureus.
Conclusion
This research conducted a comprehensive analysis of the fundamental volatile components in AKH. It subsequently elucidated the distinctions and specificities in different parts. The antibacterial effect of volatile oil in different parts was further compared, which was conducive to finding alternative parts for seeds in some active aspects, and the utilization of AKH was more sufficient.
期刊介绍：
Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.
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