pH值响应型表面功能化钛螺钉在治疗种植体相关金黄色葡萄球菌骨髓炎及生物膜形成中的疗效。

IF 10 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Hang Zhou, Youliang Ren, Kaixiong Zou, Ying Jin, Hang Liu, Haitao Jiang, Lei Shi, Xiaomin Sheng, Jason Weeks, Hannah Wang, Thomas Xue, Edward M Schwarz, Chao Xie, Zhongliang Deng, Lin Wang, Lei Chu
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引用次数: 0

摘要

植入物相关的金黄色葡萄球菌(S. aureus)骨髓炎(IASO)会在骨髓内形成葡萄球菌脓肿群,并在骨细胞裂隙-髓鞘网络(OLCN)中形成细菌定植,从而导致骨科植入物的高失败率。为解决这一问题,我们在钛螺钉上开发了负载抗菌肽(HHC36)的二氧化钛纳米管(NTs)(Ti-NTs-P-A),它整合了 pH 响应聚甲基丙烯酸,可控制 HHC36 的释放,从而消灭 IASO 中的细菌。菌落形成单位检测证实,Ti-NTs-P-A 螺钉能保持持续的抗菌效果,即使在多次更换菌液后仍能杀死 65% 以上的金黄色葡萄球菌。值得注意的是,Ti-NTs-P-A 螺钉表现出显著的 pH 响应性 HHC36 释放行为和杀菌活性,这与扫描电子显微镜观察到的多肽杀灭细菌的表型一致。转录组测序结果显示,Ti-NTs-P-A 螺钉干扰了核糖体的形成,破坏了精氨酸的生物合成,而精氨酸对细菌在酸性环境中的生存至关重要。在非感染种植体模型中,Ti-NTs-P-A 螺钉的骨与种植体接触比是临床使用的钛螺钉的 2.3 倍。在 IASO 模型中,Ti-NTs-P-A 螺钉有效地消灭了 OLCN 内的细菌,感染控制率达到 80%,并实现了理想的骨结合。总之,具有 pH 值响应抗菌特性的 Ti-NTs-P-A 螺钉在 IASO 中根除细菌和实现骨结合方面具有巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Efficacy of pH-Responsive Surface Functionalized Titanium Screws in Treating Implant-associated S. aureus Osteomyelitis with Biofilms Formation.

Implant-associated Staphylococcus aureus (S. aureus) osteomyelitis (IASO) leads to high orthopedic implant failure rates due to the formation of Staphylococcal abscess community within the bone marrow and bacterial colonization in the osteocyte lacuno-canalicular network (OLCN). To address this, antimicrobial peptides (HHC36)-loaded titania nanotubes (NTs) are developed on titanium screws (Ti-NTs-P-A), which integrate pH-responsive polymethacrylic acid to control HHC36 release for eradicating bacteria in IASO. Colony-forming unit assay confirmed that Ti-NTs-P-A screws maintained sustainable antibacterial effectiveness, killing over 65% of S. aureus even after multiple bacterial solution replacements. Notably, Ti-NTs-P-A screws exhibit significant pH-responsive HHC36 release behavior and bactericidal activity, consistent with the phenotype of peptides-killed bacteria from scanning electron microscopy. Transcriptome sequencing results reveal that Ti-NTs-P-A screws interfered with ribosome formation and disrupted the arginine biosynthesis, which is crucial for bacterial survival in acidic environments. In the non-infected implant model, the bone-implant contact ratio of the Ti-NTs-P-A screw is 2.3 times that of the clinically used titanium screw. In an IASO model, Ti-NTs-P-A screws effectively eradicated bacteria within the OLCN, achieving an 80% infection control rate and desirable osteointegration. Collectively, Ti-NTs-P-A screws with pH-responsive antibacterial properties exhibit great potential for eradicating bacteria and achieving osseointegration in IASO.

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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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