基于熵驱动催化放大系统的灵敏检测中药材中啶虫脒的色谱传感器。

IF 2.6 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Na Qin, Sunlei Yang, Rui Li, Hongyun Zhan, Lijuan Wang, Fengyun Li and Jingbo Liu
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引用次数: 0

摘要

传统的啶虫脒残留检测方法操作困难、费时费力,对检测人员的专业知识要求较高,不能满足现场快速检测的要求。为此,研究人员开发了一种基于熵催化放大系统的比色适配传感器,用于超灵敏、高选择性地检测啶虫脒。在没有啶虫脒的情况下,适配体和 cDNA 形成双链结构。形成的 hemin/G-quadruplex 模仿 DNA 酶能在 H2O2 的帮助下催化底物 ABTS 生成有色离子 ABTS-,溶液变成蓝绿色。相反,啶虫脒的存在会引发 cDNA 的释放,进而启动熵驱动系统,导致无法形成 DNA 酶,因此溶液中不会产生蓝绿色。啶虫脒的数量决定了颜色的深浅。在最佳实验条件下,该方法在 0.1-100 ng/mL 浓度范围内检测啶虫脒呈线性相关(R2 = 0.9837),检出限为 0.06 ng/mL。用所建立的方法测定了加标薏苡仁和苦杏仁中的啶虫脒含量,回收率为90.3-110.3%。该方法无酶、无标记,是一种简单、灵敏、快速的检测平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Aptasensor based on entropy-driven catalytic amplification system for the sensitive detection of acetamiprid in Chinese herbal medicine†

Aptasensor based on entropy-driven catalytic amplification system for the sensitive detection of acetamiprid in Chinese herbal medicine†

The traditional method of acetamiprid residue detection is difficult to operate, time-consuming, laborious and requires high professional knowledge of the detection personnel, which cannot meet the requirement of on-field rapid detection. For this reason, a colorimetric aptasensor based on an entropy-catalyzed amplification system was developed for the ultrasensitive and selective determination of acetamiprid. In the absence of acetamiprid, the aptamer and cDNA form a double-stranded structure. The formed hemin/G-quadruplex mimicking DNAzyme can catalyze the substrate ABTS to generate the colored ion ABTS with the help of H2O2, and the solution turns blue-green. On the contrary, the presence of acetamiprid triggers the release of cDNA, which in turn initiates the entropy-driven system, resulting in the inability to form DNAzyme and therefore no blue-green color production in the solution. The quantity of acetamiprid determines the color. Under the optimal experimental conditions, the method showed a linear correlation (R2 = 0.9837) for the detection of acetamiprid in the concentration range of 0.1–100 ng/mL, with a limit of detection of 0.06 ng/mL. The developed method was used for the determination of acetamiprid in spiked Coix lacryma and Bitter almond, with recoveries in the range of 90.3–110.3%. The proposed enzyme-free and label-free assay can be developed into a simple, sensitive and rapid detection platform.

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来源期刊
Analytical Methods
Analytical Methods CHEMISTRY, ANALYTICAL-FOOD SCIENCE & TECHNOLOGY
CiteScore
5.10
自引率
3.20%
发文量
569
审稿时长
1.8 months
期刊介绍: Early applied demonstrations of new analytical methods with clear societal impact
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