Na Qin, Sunlei Yang, Rui Li, Hongyun Zhan, Lijuan Wang, Fengyun Li and Jingbo Liu
{"title":"基于熵驱动催化放大系统的灵敏检测中药材中啶虫脒的色谱传感器。","authors":"Na Qin, Sunlei Yang, Rui Li, Hongyun Zhan, Lijuan Wang, Fengyun Li and Jingbo Liu","doi":"10.1039/D4AY01655F","DOIUrl":null,"url":null,"abstract":"<p >The traditional method of acetamiprid residue detection is difficult to operate, time-consuming, laborious and requires high professional knowledge of the detection personnel, which cannot meet the requirement of on-field rapid detection. For this reason, a colorimetric aptasensor based on an entropy-catalyzed amplification system was developed for the ultrasensitive and selective determination of acetamiprid. In the absence of acetamiprid, the aptamer and cDNA form a double-stranded structure. The formed hemin/G-quadruplex mimicking DNAzyme can catalyze the substrate ABTS to generate the colored ion ABTS<small><sup>−</sup></small> with the help of H<small><sub>2</sub></small>O<small><sub>2</sub></small>, and the solution turns blue-green. On the contrary, the presence of acetamiprid triggers the release of cDNA, which in turn initiates the entropy-driven system, resulting in the inability to form DNAzyme and therefore no blue-green color production in the solution. The quantity of acetamiprid determines the color. Under the optimal experimental conditions, the method showed a linear correlation (<em>R</em><small><sup>2</sup></small> = 0.9837) for the detection of acetamiprid in the concentration range of 0.1–100 ng/mL, with a limit of detection of 0.06 ng/mL. The developed method was used for the determination of acetamiprid in spiked Coix lacryma and Bitter almond, with recoveries in the range of 90.3–110.3%. The proposed enzyme-free and label-free assay can be developed into a simple, sensitive and rapid detection platform.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 2","pages":" 223-231"},"PeriodicalIF":2.6000,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Aptasensor based on entropy-driven catalytic amplification system for the sensitive detection of acetamiprid in Chinese herbal medicine†\",\"authors\":\"Na Qin, Sunlei Yang, Rui Li, Hongyun Zhan, Lijuan Wang, Fengyun Li and Jingbo Liu\",\"doi\":\"10.1039/D4AY01655F\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >The traditional method of acetamiprid residue detection is difficult to operate, time-consuming, laborious and requires high professional knowledge of the detection personnel, which cannot meet the requirement of on-field rapid detection. For this reason, a colorimetric aptasensor based on an entropy-catalyzed amplification system was developed for the ultrasensitive and selective determination of acetamiprid. In the absence of acetamiprid, the aptamer and cDNA form a double-stranded structure. The formed hemin/G-quadruplex mimicking DNAzyme can catalyze the substrate ABTS to generate the colored ion ABTS<small><sup>−</sup></small> with the help of H<small><sub>2</sub></small>O<small><sub>2</sub></small>, and the solution turns blue-green. On the contrary, the presence of acetamiprid triggers the release of cDNA, which in turn initiates the entropy-driven system, resulting in the inability to form DNAzyme and therefore no blue-green color production in the solution. The quantity of acetamiprid determines the color. Under the optimal experimental conditions, the method showed a linear correlation (<em>R</em><small><sup>2</sup></small> = 0.9837) for the detection of acetamiprid in the concentration range of 0.1–100 ng/mL, with a limit of detection of 0.06 ng/mL. The developed method was used for the determination of acetamiprid in spiked Coix lacryma and Bitter almond, with recoveries in the range of 90.3–110.3%. The proposed enzyme-free and label-free assay can be developed into a simple, sensitive and rapid detection platform.</p>\",\"PeriodicalId\":64,\"journal\":{\"name\":\"Analytical Methods\",\"volume\":\" 2\",\"pages\":\" 223-231\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-11-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Methods\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/ay/d4ay01655f\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/ay/d4ay01655f","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Aptasensor based on entropy-driven catalytic amplification system for the sensitive detection of acetamiprid in Chinese herbal medicine†
The traditional method of acetamiprid residue detection is difficult to operate, time-consuming, laborious and requires high professional knowledge of the detection personnel, which cannot meet the requirement of on-field rapid detection. For this reason, a colorimetric aptasensor based on an entropy-catalyzed amplification system was developed for the ultrasensitive and selective determination of acetamiprid. In the absence of acetamiprid, the aptamer and cDNA form a double-stranded structure. The formed hemin/G-quadruplex mimicking DNAzyme can catalyze the substrate ABTS to generate the colored ion ABTS− with the help of H2O2, and the solution turns blue-green. On the contrary, the presence of acetamiprid triggers the release of cDNA, which in turn initiates the entropy-driven system, resulting in the inability to form DNAzyme and therefore no blue-green color production in the solution. The quantity of acetamiprid determines the color. Under the optimal experimental conditions, the method showed a linear correlation (R2 = 0.9837) for the detection of acetamiprid in the concentration range of 0.1–100 ng/mL, with a limit of detection of 0.06 ng/mL. The developed method was used for the determination of acetamiprid in spiked Coix lacryma and Bitter almond, with recoveries in the range of 90.3–110.3%. The proposed enzyme-free and label-free assay can be developed into a simple, sensitive and rapid detection platform.