圆柱桑叶和荚果的水醇提取物对 Wistar 大鼠的抗氧化、抗 AChE 和神经保护作用

IF 3.4 Q2 PHARMACOLOGY & PHARMACY
Sunil Shewale, Vaishali Undale, Vrushali Bhalchim, Mohini Kuchekar, Bhagyashri Warude, Mayur Mali
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引用次数: 0

摘要

神经退行性疾病的发病率不断上升,是全球关注的重大健康问题。这类疾病的预后往往不佳,患者需要支持性治疗才能有效控制病情。本研究调查了水醇提取物对 Wistar 大鼠的抗氧化、抗乙酰胆碱酯酶(anti-AChE)作用。植物经鉴定后进行了药理评估。通过东莨菪碱诱导的 Wistar 大鼠氧化应激和乙酰胆碱水平的变化来评估其抗氧化和抗乙酰胆碱酯酶活性。在研究期间的第 0 天、第 7 天和第 14 天,对大鼠的行为参数进行了评估,包括开阔地测试、新物体识别测试和莫里斯水迷宫任务。在治疗 7 天后,还对脑匀浆中的不同抗氧化酶进行了评估。治疗 14 天后,对大鼠大脑中的 AChE 水平进行了评估。最后,对大脑样本进行了组织病理学检查。由于含有次生代谢物,Sansevieria cylindrica 和 Plumeria obtusa 具有很强的抗氧化和抗 AChE 活性。这两种植物的高剂量组合对大鼠的饲养和交配有重大影响(p < 0.05)。在所有剂量下,圆筒形三叶草单独使用或与钝叶梅混合使用都能显著降低大鼠的逃逸潜伏期(p <0.05)。在 1:1 的植物提取物组合中,脑脂质过氧化水平明显低于标准。不同浓度的提取物对 GSH、CAT 和 SOD 的水平均有明显的抑制作用(分别为 p < 0.05 和 p < 0.001)。与疾病对照组相比,两种植物的高剂量组合使雄性和雌性大鼠的 AChE 水平分别降低了约 34.62% 和 31.10%。这一点在脑组织病理学中表现得更为明显。所有体内研究结果都得到了体外自由基清除效果和实验室研究的支持。从梅花荚果和圆柱桑叶中提取的水醇提取物具有很强的天然抗氧化和乙酰胆碱酯酶抑制作用,可以帮助治疗包括阿尔茨海默氏症在内的神经退行性疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-oxidative, anti-AChE and neuroprotective effect of hydro-alcoholic extract of Sansevieria cylindrica leaves and Plumeria obtusa seed pods in Wistar rats

The rising incidence of neurodegenerative diseases represents a significant global health concern. The prognosis for such diseases is often unfavorable, and patients require supportive treatment to manage their condition effectively. The present study investigated the anti-oxidative, anti-acetylcholinesterase (anti-AChE) effect of hydro-alcoholic extract of Sansevieria cylindrica leaves and Plumeria obtusa seed pods in Wistar rats. Plants were authenticated followed by pharmacognostic evaluation. The antioxidant and anti-AChE activities were evaluated by scopolamine-induced oxidative stress and acetylcholine level alterations in Wistar rats. On the 0th, 7th, and 14th days during study period behavioral parameters were evaluated, including open field test, new object recognition test, and Morris water maze task. Different antioxidant enzymes were also evaluated in brain homogenate post 7 days treatment. AChE levels in rats’ brains were assessed post 14 days of treatment. Finally, histopathology of brains samples was performed. Correspondingly, in-vitro and in-silico studies were done to support the study findings.

Sansevieria cylindrica and Plumeria obtusa exhibited strong antioxidant and anti-AChE activities due to secondary metabolites content. Rearing and, line crossing by rats showed substantial alteration (p < 0.05) by combination of both plants at high dose. The escape latency found to be reduced significantly (p < 0.05) by Sansevieria cylindrica individually and in combination with Plumeria obtusa at all doses. The lipid peroxidation level in brain was decreased significantly than standard at 1:1 combination of plants extracts. The levels of GSH, CAT and SOD were attenuated significantly by different concentration of extracts (p < 0.05 and p < 0.001, respectively). The reduction in AChE levels was around 34.62% and 31.10% in male and female rats, respectively in combination of both plants at high dose compared to disease control group. It was further evident in brain histopathology. All in-vivo results were supported by in-vitro free radicals scavenging effect and in-silico study.

The hydro-alcoholic extracts from Plumeria obtusa seed pods and Sansevieria cylindrica leaves showed a strong natural antioxidant, and acetylcholinesterase inhibitions which can aid in the treatment of neurodegerative diseases including Alzheimer.

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来源期刊
自引率
0.00%
发文量
44
审稿时长
23 weeks
期刊介绍: Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.
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