大豆根瘤菌属在培养过程中自发产生丰富多样的温带噬菌体。

IF 3.8 3区 医学 Q2 VIROLOGY
Viruses-Basel Pub Date : 2024-11-07 DOI:10.3390/v16111750
Vanessa A Richards, Barbra D Ferrell, Shawn W Polson, K Eric Wommack, Jeffry J Fuhrmann
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引用次数: 0

摘要

大豆根瘤菌(Bradyrhizobium spp.)是一种共生根瘤菌,可为寄主植物固定大气中的氮。特拉华大学根瘤双歧杆菌培养物保藏中心(UDBCC;353 个品系)的建立是为了研究大豆根瘤双歧杆菌的多样性和生态学。一些 UDBCC 菌株在常规培养条件下会自发产生温带(溶解性)噬菌体,而不需要化学或其他明显的诱导剂。噬菌体的自发产生可能会促进横向基因转移,形成细菌基因组和相关表型。使用外荧光显微镜检查了 UDBCC 的不同子集(n = 98)自发产生的病毒样颗粒(VLPs),其中大多数(69%)在实验室培养中产生可检测到的 VLPs(>1 × 107 mL-1)。利用透射电子显微镜对高产菌株(>2.0 × 108 VLP mL-1; n = 44)的噬菌体进行了检测。观察到的形态多种多样,包括不同的尾部类型和长度、噬菌体大小和形状,以及是否存在衣领或基板。在许多情况下,还观察到与病毒大小相似的细胞外囊泡。在所研究的四个物种中,有三个(日本疫杆菌、麋鹿疫杆菌和 B. diazoefficiens)产生了明显的无尾噬菌体。除 B. ottawaense 外,其他所有物种都产生了类似虹吸病毒的噬菌体,而除了 B. diazoefficiens 外,其他所有物种都产生了类似荚膜病毒的噬菌体。肌病毒样噬菌体仅限于日本酵母菌(B. japonicum)和麋鹿酵母菌(B. elkanii)。至少有三个菌株是多源的,可产生三种不同的形态。这些观察结果表明,自发产生的噬菌体可能在大豆根瘤菌的生态学和进化中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Soybean Bradyrhizobium spp. Spontaneously Produce Abundant and Diverse Temperate Phages in Culture.

Soybean bradyrhizobia (Bradyrhizobium spp.) are symbiotic root-nodulating bacteria that fix atmospheric nitrogen for the host plant. The University of Delaware Bradyrhizobium Culture Collection (UDBCC; 353 accessions) was created to study the diversity and ecology of soybean bradyrhizobia. Some UDBCC accessions produce temperate (lysogenic) bacteriophages spontaneously under routine culture conditions without chemical or other apparent inducing agents. Spontaneous phage production may promote horizontal gene transfer and shape bacterial genomes and associated phenotypes. A diverse subset (n = 98) of the UDBCC was examined for spontaneously produced virus-like particles (VLPs) using epifluorescent microscopy, with a majority (69%) producing detectable VLPs (>1 × 107 mL-1) in laboratory culture. Phages from the higher-producing accessions (>2.0 × 108 VLP mL-1; n = 44) were examined using transmission electron microscopy. Diverse morphologies were observed, including various tail types and lengths, capsid sizes and shapes, and the presence of collars or baseplates. In many instances, putative extracellular vesicles of a size similar to virions were also observed. Three of the four species examined (B. japonicum, B. elkanii, and B. diazoefficiens) produced apparently tailless phages. All species except B. ottawaense also produced siphovirus-like phages, while all but B. diazoefficiens additionally produced podovirus-like phages. Myovirus-like phages were restricted to B. japonicum and B. elkanii. At least three strains were polylysogens, producing up to three distinct morphotypes. These observations suggest spontaneously produced phages may play a significant role in the ecology and evolution of soybean bradyrhizobia.

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来源期刊
Viruses-Basel
Viruses-Basel VIROLOGY-
CiteScore
7.30
自引率
12.80%
发文量
2445
审稿时长
1 months
期刊介绍: Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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